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LipoKnoxa™ Human CEP19 shRNA Ad5 Particle (Silencing)
Cat. No.:
V0126XX28
Species:
Human
Target Gene:
CEP19
Vector System:
Adenovirus
Modulation Type:
Silencing (shRNA)
SPECIFIC INQUIRY
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| Sub Cat. No. | TargetSeq | Region | Inquiry |
|---|---|---|---|
| V0126XX28-1 | GTTTCAGCCTCCAGCTATTAT | CDS | Inquiry |
| V0126XX28-2 | CCAGAGCTGCTGAACAATTAA | CDS | Inquiry |
| V0126XX28-3 | TGGGAAATCCACCCATAATAA | 3' UTR | Inquiry |
| V0126XX28-4 | Other | Inquiry |
Product Overview
Description:
LipoKnoxa™ Human CEP19 shRNA Ad5 Particle (Silencing) enables the targeted inhibition of CEP19, a ciliary protein associated with morbid obesity and insulin resistance. Utilizing a miR30 or U6-based system, this product facilitates the study of basal body function in metabolism. All particles are screened through comprehensive QC protocols to ensure the repeatability of results in cellular and molecular biology.
Production Cell Line:
HEK293
Viral Backbone:
Adenovirus type 5 (dE1/E3)
Promoter:
U6; CMV; EF1α; CAG; UBC
Product Availability:
Produced Upon Order
Specification
Titer Test:
qPCR
Insert Verification:
All viral preparations are validated via Sequencing and PCR to ensure 100% sequence identity and the structural integrity of the vector genomes.
Sterility Test:
This product has been certified sterile following comprehensive microbial growth analysis, confirming the absence of bacterial and fungal contamination.
Mycoplasma Test:
This product was certified negative for mycoplasma contamination following stringent QC analysis, ensuring the absence of all mycoplasmal agents.
Other QC:
Beyond standard protocols, we offer customized knockdown efficiency validation through in vitro and in vivo assessments. This includes precise analysis of mRNA/protein reduction and subsequent biological responses to ensure the functional potency of the shRNA-mediated gene silencing.
Storage:
Upon receipt, viral preparations should be immediately transferred to -80°C for long-term storage to ensure maximum stability and maintain product integrity.
Stability:
This product maintains excellent biological activity for 6–12 months (and up to 2 years in specific cases) when stored continuously at -80°C. Once thawed, the working solution remains stable for 2–3 weeks at 4°C without significant loss of viral potency.
Shipping Condition:
All viral preparations are shipped on dry ice to ensure maximum biological activity and stability during transit.
Handling Notes:
Viral particles are susceptible to temperature fluctuations and freeze-thaw cycles. To preserve functional titers, it is essential to aliquot the vector into low-protein-binding tubes immediately upon first thaw. To ensure experimental success and biological safety, all procedures must be conducted within a certified biosafety cabinet.
Intended Use:
This product is intended for research use only and is not for use in diagnosis or therapeutic applications.
Product Disclaimer:
While our products are committed to excellence through rigorous internal QC inspections, we cannot guarantee specific performance or experimental outcomes due to the inherent complexity of biological systems. Users assume full responsibility for product storage, handling, and strict compliance with all applicable safety protocols, biosafety requirements, and legal regulations during all operational processes.
Target Profile
Gene Name:
CEP19
Full Name:
Centrosomal protein 19
Gene Symbol:
MOSPGF; C3orf34
Gene ID:
84984
RefSeq ID-1:
NP_001366398.1
RefSeq ID-2:
NM_001379469.1
Summary:
CEP19 encodes a centrosomal protein that localizes to centrosomes and primary cilia and co-localizes with markers of the mother centriole. The gene is located within a chromosomal region on human chromosome 3 that has been linked to severe obesity. Functional studies in mice demonstrate that loss of the CEP19 ortholog leads to morbid obesity, excessive food intake, glucose intolerance, and insulin resistance. In humans, pathogenic mutations in CEP19 cause morbid obesity and spermatogenic failure, emphasizing its role in metabolic and reproductive biology. A pseudogene corresponding to CEP19 is present on chromosome 2.
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