Product Overview
Description:
AdipoUpX™ Human FAS Ad5 Adenoviral Particle (Overexpression) is a ready-to-use Ad5 adenovirus vector particle designed for the efficient transient overexpression of the human FAS gene. It enables researchers to study programmed cell death (apoptosis) and its role in tissue remodeling and inflammatory cell recruitment during metabolic stress. The product is supported by flexible reporter gene selection and rigorous quality control (titer, sterility, and mycoplasma detection).
Production Cell Line:
HEK293
Viral Backbone:
Adenovirus type5 (dE1/E3)
Promoter:
CMV
Product Availability:
Produced Upon Order
Specification
Titer Test:
qPCR
Insert Verification:
All viral preparations have been validated via PCR to confirm the integrity of their vector genomes.
Sterility Test:
This product was confirmed to be sterile upon testing.
Mycoplasma Test:
This product has passed quality control, confirming that it is free of mycoplasma contamination.
Other QC:
We perform customized supplementary testing and in vitro/in vivo transduction assessments based on specific project requirements to analyze the expression levels and biological functions of transgenes.
Storage:
Upon receipt of viral preparations, they must be immediately transferred to a -80°C environment for long-term storage.
Stability:
The product guarantees excellent stability for 6-12 months when stored continuously at -80°C (and in some cases, up to 2 years). Upon thawing, the working solution is refrigerated at 4°C for up to 2-3 weeks without significant loss of biological activity.
Shipping Condition:
Our adenovirus viral products are shipped using dry ice.
Handling Notes:
Viral particles are highly sensitive to temperature fluctuations and freeze-thaw cycles. To maintain titer and avoid repeated freeze-thaw cycles, it is recommended to aliquot the vector immediately upon receipt into low-protein-binding tubes and store according to specified conditions. Ensure all operations are performed within a biosafety cabinet.
Intended Use:
This product is intended for research use only and is not for use in diagnosis or therapeutic applications.
Product Disclaimer:
Our company is committed to product quality, and all products have undergone rigorous internal QC inspections. Given the diversity and complexity of experimental conditions, we do not guarantee product performance or experimental results in any specific application. Users bear ultimate responsibility for product storage, handling, and compliance with all safety protocols, laws, regulations, and biosafety requirements throughout all operational processes.
Target Profile
Gene Name:
FAS
Full Name:
Fas cell surface death receptor
Gene Symbol:
APT1; CD95; FAS1; APO-1; FASTM; ALPS1A; TNFRSF6
Summary:
FAS encodes a death receptor belonging to the TNF receptor superfamily and contains an intracellular death domain essential for apoptosis signaling. Engagement of FAS by its ligand induces formation of a death-inducing signaling complex comprising FADD, caspase-8, and caspase-10, leading to activation of a caspase cascade and programmed cell death. Beyond apoptosis, FAS signaling can activate NF-κB and MAPK pathways, including ERK1 and JNK, and participates in proliferative signaling in fibroblasts and T cells. In obesity, altered FAS-mediated apoptosis contributes to adipose tissue inflammation and immune cell turnover. Multiple splice variants exist, including soluble forms that may dampen apoptotic signaling.
