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Human adiponectin (ADP) ELISA kit (0.78-50 μg/mL)
Cat. No.:
OB0525WXX-207
Assay Type:
Quantitative sandwich ELISA
Target Species:
Human
Assay Target:
ADP
Size:
Add to basket
Product Overview
Description:
Human adiponectin (ADP) ELISA kit (0.78-50 μg/mL) is a ready-to-use ELISA kit for analyzing human ADP levels with a detection range of 0.78-50 μg/mL. It is an efficient dynamic research tool.
Assay Principle:
The assay utilizes a two-site sandwich ELISA for the quantitative detection of ADP in human serum and plasma. An antibody specific for ADP is pre-coated on a microtiter plate. Standards and samples are pipetted into the wells, and any ADP will bind to the immobilized antibody. After removing unbound material, a biotin-conjugated antibody specific for ADP is added to the wells. After washing, streptavidin-conjugated HRP is added to the wells. After washing to remove unbound avidin-enzyme reagent, a substrate solution is added to the wells, and the color is developed in proportion to the amount of ADP bound in the first step. The color development is stopped, and the color intensity is measured.
Background:
ADP is an important adipokine involved in the regulation of fat metabolism and insulin sensitivity, exhibiting antidiabetic, anti-atherosclerotic, and anti-inflammatory properties. It increases glucose utilization and fatty acid burning, stimulates AMPK phosphorylation and activation in liver and skeletal muscle, and may play a role in cell growth, angiogenesis, and tissue remodeling.
Synonyms:
ADIPOQ; ACDC; APM1; Adipocyte C1q and collagen domain-containing protein; ACRP30; Adipose most abundant gene transcript 1 protein; apM-1; Gelatin-binding protein; Adiponectin
Applications:
Human adiponectin (ADP) ELISA kit (0.78-50 μg/mL) is designed for the in vitro quantitative analysis of ADP levels in human serum, plasma, and other biological fluid samples.
Research Area:
Hormone research; Lipid metabolism; Cell differentiation; Obesity
Specification
Sample Type:
serum; Plasma; Other biological fluids
Detection Range:
0.78-50 μg/mL
Precision (Intra-assay):
CV<8%
Precision (Inter-assay):
CV<12%
Cross-reactivity:
No significant cross-reactivity or interference was observed.
Storage:
Store at 2-8°C.
