Rat leptin receptor (LEPR) ELISA kit (0.5-150 ng/mL)

Cat. No.: 0126WXX-978

Assay Type: Quantitative sandwich ELISA

Target Species: Rat

Assay Target: LEPR

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Rat leptin receptor (LEPR) ELISA kit (0.5-150 ng/mL)

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Product Overview

Description: Rat leptin receptor (LEPR) ELISA kit (0.5-150 ng/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of LEPR in rat samples with a range of 0.5-150 ng/mL and a minimum detectable dose (sensitivity) of 0.25 ng/mL. The kit is highly sensitive and easy to use.

Assay Principle: The microplate is pre-coated with a rat LEPR antibody. LEPR in the sample binds to the immobilized antibodies, followed by the addition of a biotinylated rat LEPR detection antibody and streptavidin-HRP. Following incubation and a wash step, a substrate solution is added, and color develops in proportion to the amount of rat LEPR. The reaction is terminated with an acidic stop solution, and the final absorbance is measured at 450 nm.

Background: LEPR, as a peptide hormone receptor, is responsible for receiving leptin signals. When leptin binds to LEPR, it initiates a series of intracellular cascades, including the positive regulation of the JAK-STAT signaling pathway and the positive regulation of the PI3K signaling pathway. These pathways are crucial for translating leptin signals into biological responses, such as appetite suppression and metabolic regulation. The relationship between LEPR and obesity is paramount. When leptin functions as the “satiety hormone,” LEPR acts as the “satiety switch” in the brain and other tissues. Without functional receptors, leptin signals cannot be received regardless of how high leptin concentrations become.

Synonyms: CD295; LEPR; OBR; LR

Applications: Rat leptin receptor (LEPR) ELISA kit (0.5-150 ng/mL) is used to quantify LEPR in serum, plasma, cell culture supernatant, cell lysates, and tissue homogenates of rat, providing data to support research in a wide range of areas, including signal transduction, CD & adhesion molecule, metabolic pathway, endocrinology, obesity, etc.

Research Area: Signal transduction; CD & Adhesion molecule; Metabolic pathway; Endocrinology; Obesity

Specification

Sample Type: Serum; Plasma; Cell culture supernatants; Cell lysates; Tissue homogenates

Detection Range: 0.5-150 ng/mL

Sensitivity: 0.25 ng/mL

Precision (Intra-assay): CV<8%

Precision (Inter-assay): CV<10%

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at 2-8°C.

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