Canine glutathione peroxidase (GSH-Px) ELISA kit-Quantitative competitive

Cat. No.: 0126WXX-302

Assay Type: Quantitative competitive ELISA

Target Species: Canine

Assay Target: GSH-Px

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Canine glutathione peroxidase (GSH-Px) ELISA kit-Quantitative competitive

INQUIRY Datasheet MSDS

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Product Overview

Description: Canine glutathione peroxidase (GSH-Px) ELISA kit is an ELISA-based in vitro research tool designed specifically for the quantitative detection of GSH-Px in canine samples with a minimum detectable dose (sensitivity) of 0.1 ng/mL. The kit is highly sensitive and easy to use.

Assay Principle: This GSH-Px ELISA kit utilizes a competitive enzyme immunoassay technique featuring a polyclonal anti-GSH-Px antibody and a GSH-Px-HRP conjugate. Samples and assay buffer are incubated with the GSH-Px-HRP conjugate in a pre-coated microplate for one hour. During this process, endogenous GSH-Px from the sample competes with the GSH-Px-HRP conjugate for a limited number of antibody binding sites. After the incubation period, the wells are washed five times. An HRP substrate is then added, forming a blue complex that turns yellow upon the addition of a stop solution. The absorbance is measured at 450 nm, where the color intensity is inversely proportional to the GSH-Px concentration. A standard curve is generated to relate the O.D. to standard concentrations, from which the GSH-Px level in each sample is interpolated.

Background: Glutathione peroxidase is a family of antioxidant enzymes widely distributed throughout the human body. Their core function is to protect cells from oxidative damage. Obesity itself is considered a chronic low-grade inflammatory state, often accompanied by significant oxidative stress. Glutathione peroxidase is a potential regulator of obesity.

Synonyms: GPXP; GPx-3; GSHPx-3; GPx-P; GSHPx-P; GPX3

Applications: Canine glutathione peroxidase (GSH-Px) ELISA kit is used to quantify GSH-Px in serum, plasma, cell culture supernatants, body fluid, and tissue homogenate of canine, providing data to support research in a wide range of areas, including oxidative stress, metabolism, obesity, and others.

Research Area: Oxidative stress; Metabolism; Obesity

Specification

Sample Type: Serum; Plasma; Cell culture supernatants; Body fluid; Tissue homogenate

Detection Range: 100-2500 pg/mL

Sensitivity: 0.1 ng/mL

Precision (Intra-assay): CV<10%

Precision (Inter-assay): CV<12%

Cross-reactivity: No significant cross-reactivity or interference was observed.

Recovery: 92-101%

Storage: Store at 2-8°C.

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