Human peptide YY (PYY) ELISA kit-Quantitative sandwich (6.25-400 pg/mL)

Cat. No.: 0126WXX-1657

Assay Type: Quantitative sandwich ELISA

Target Species: Human

Assay Target: PYY

Size:

Human peptide YY (PYY) ELISA kit-Quantitative sandwich (6.25-400 pg/mL)

INQUIRY Datasheet MSDS

Add to basket

Product Overview

Description: Human peptide YY (PYY) ELISA kit-Quantitative sandwich (6.25-400 pg/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of PYY in human samples with a range of 6.25-400 pg/mL and a minimum detectable dose (sensitivity) of 3.1 pg/mL. The kit is highly sensitive and easy to use.

Assay Principle: This ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate is pre-coated with an antibody specific to PYY. During the assay, PYY in the sample or standard competes with a fixed amount of biotin-labeled PYY for binding sites on the immobilized monoclonal antibody. After washing the plate to remove excess conjugate and unbound samples, Avidin-conjugated HRP is added. Following incubation, TMB substrate is introduced. The reaction is stopped with sulphuric acid, and the color change is measured at 450 nm ± 2 nm. The concentration of PYY is inversely proportional to the O.D. value.

Background: PYY (Peptide YY), a critical gut peptide and member of the NPY family located at chromosomal position 17q21.1, serves as a fundamental regulator in the physiological landscape of obesity and digestive homeostasis. Secreted into the extracellular region as two distinct isoforms through alternative splicing, this neuropeptide hormone exerts its biological influence primarily through G-protein coupled receptor signaling pathways to modulate eating behavior and appetite regulation. Its multifaceted role in the gastrointestinal tract includes the inhibition of exocrine pancreatic secretion, the mediation of vasoconstrictory actions, and the suppression of jejunal and colonic motility, thereby slowing digestion to enhance satiety. Beyond its classical endocrine functions in feeding behavior, PYY is also implicated in broader cellular processes such as cell proliferation and cytoskeleton organization.

Synonyms: Peptide YY-like

Applications: Human peptide YY (PYY) ELISA kit-Quantitative sandwich (6.25-400 pg/mL) is used to quantify PYY in human samples, providing data to support research in a wide range of areas, including neuroscience, hepatology, obesity, and others.

Research Area: Neuro science; Hepatology; Obesity

Specification

Sample Type: Human samples

Detection Range: 6.25-400 pg/mL

Sensitivity: 3.1 pg/mL

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at -20°C.

Related Products

Inquiry

Inquiry Basket

Delete selected Check Out