Human peroxisome proliferator-activated receptor delta (PPARδ) ELISA kit (31.2-2000 pg/mL)

Cat. No.: 0126WXX-1503

Assay Type: Quantitative sandwich ELISA

Target Species: Human

Assay Target: PPARδ

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Human peroxisome proliferator-activated receptor delta (PPARδ) ELISA kit (31.2-2000 pg/mL)

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Product Overview

Description: Human peroxisome proliferator-activated receptor delta (PPARδ) ELISA kit (31.2-2000 pg/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of PPARδ in human samples with a range of 31.2-2000 pg/mL and a minimum detectable dose (sensitivity) of 10 pg/mL. The kit is highly sensitive and easy to use.

Assay Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to PPARδ. Standards or samples are introduced into the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for PPARδ. Avidin conjugated to HRP is then added to each microplate well and incubated. Following this, a TMB substrate solution is added to each well. Only those wells that contain PPARδ, biotin-conjugated antibody, and enzyme-conjugated Avidin will exhibit a colorimetric change. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution, and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of PPARδ in the samples is determined by comparing the O.D. of the samples to the standard curve.

Background: Peroxisome proliferator-activated receptor delta (PPARδ) is a ligand-activated nuclear receptor that regulates gene expression involved in fatty acid transport, beta-oxidation, and lipid metabolism. Activated by polyunsaturated fatty acids and other ligands, PPARδ promotes energy expenditure, fatty acid catabolism, and transcription of metabolic genes, contributing to the regulation of glucose and lipid homeostasis. Dysregulation of PPARδ activity can impair lipid metabolism and energy balance, linking it to obesity and related metabolic disorders.

Synonyms: PPARD; PPARd; PPAR-delta; Nuclear hormone receptor 1; NUC1

Applications: Human peroxisome proliferator-activated receptor delta (PPARδ) ELISA kit (31.2-2000 pg/mL) is used to quantify PPARδ in human samples, providing data to support research in a wide range of areas, including signal transduction, metabolic pathways, obesity, and others.

Research Area: Signal transduction; Metabolic pathway; Obesity

Specification

Sample Type: Human samples

Detection Range: 31.2-2000 pg/mL

Sensitivity: 10 pg/mL

Precision (Intra-assay): CV≤5.6%

Precision (Inter-assay): CV≤9.6%

Cross-reactivity: No significant cross-reactivity or interference was observed.

Recovery: 1.05

Storage: Store at -20°C.

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