Sheep insulin like growth factor binding protein 2 (IGFBP-2) ELISA kit-Quantitative competitive

Cat. No.: 0126WXX-495

Assay Type: Quantitative competitive ELISA

Target Species: Sheep

Assay Target: IGFBP-2

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Sheep insulin like growth factor binding protein 2 (IGFBP-2) ELISA kit-Quantitative competitive

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Product Overview

Description: Sheep insulin like growth factor binding protein 2 (IGFBP-2) ELISA kit is an ELISA-based in vitro research tool designed specifically for the quantitative detection of IGFBP-2 in sheep samples with a minimum detectable dose (sensitivity) of 1.0 ng/mL. The kit is highly sensitive and easy to use.

Assay Principle: The IGFBP-2 ELISA kit applies a competitive enzyme immunoassay technique utilizing an anti-IGFBP-2 antibody and an IGFBP-2-HRP conjugate. Samples and buffer are incubated with the conjugate in a pre-coated plate for one hour. During this time, endogenous IGFBP-2 competes with the IGFBP-2-HRP conjugate for limited antibody binding sites. After washing the wells five times, an HRP substrate is added to form a blue complex, which turns yellow upon the addition of the stop solution. The absorbance is measured at 450 nm. The color intensity is inversely proportional to the IGFBP-2 concentration.

Background: IGFBP-2 is a multifunctional molecule that plays a crucial role in human growth, metabolism, and cellular processes by regulating the biological activity of insulin-like growth factors (IGFs). Primarily through binding to IGF proteins, IGFBP-2 prolongs their half-life in the body and modulates their interactions with cell surface receptors, thereby inhibiting or enhancing the pro-growth effects of IGFs. The role of IGFBP-2 in obesity has garnered increasing attention. Its reduced levels are closely associated with obesity, dyslipidemia, and insulin resistance.

Synonyms: IBP-2; IGF-binding protein 2; IGFBP2; BP2, IBP2; Insulin-Like Growth Factor Binding Protein 2, 36kDa; Insulin-Like Growth Factor-Binding Protein 2

Applications: Sheep insulin like growth factor binding protein 2 (IGFBP-2) ELISA kit is used to quantify IGFBP-2 in serum, plasma, cell culture supernatants, body fluid, and tissue homogenate of sheep, providing data to support research in a wide range of areas, including immunity, endocrinology, signal transduction, obesity, and others.

Research Area: Immunity; Endocrinology; Signal transduction; Obesity

Specification

Sample Type: Serum; Plasma; Cell culture supernatants; Body fluid; Tissue homogenate

Detection Range: 25-500 ng/mL

Sensitivity: 1.0 ng/mL

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at 2-8°C.

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