Rat cytochrome C (CYCS) ELISA kit

Cat. No.: OB0625WXX-551

Assay Type: Quantitative sandwich ELISA

Target Species: Rat

Assay Target: CYCS

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Product Overview

Description: Rat cytochrome C (CYCS) ELISA kit is an ELISA-based in vitro research tool designed specifically for the quantitative detection of CYCS in rat with a range of 0.312-20 ng/mL and a specificity of 0.112 ng/mL.

Assay Principle: The microtiter plates in this kit are pre-coated with an antibody specific for CYCS. Standards or samples are then added to the appropriate microtiter plate wells along with a biotin-conjugated antibody specific for CYCS. Avidin conjugated to horseradish peroxidase (HRP) is then added to each microplate well and incubated. Upon addition of the TMB substrate solution, only the wells containing CYCS, biotin-conjugated antibody, and enzyme-conjugated avidin will show a color change. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution, and the color change is then measured spectrophotometrically at 450 nm ± 10 nm. The concentration of CYCS in the sample is then determined by comparing the OD of the sample to a standard curve.

Background: CYCS is a mitochondrial protein that plays a vital role in the electron transport chain for ATP production and, upon release into the cytoplasm, initiates apoptosis.

Synonyms: CYC; HCS; THC4; Cytochrome c, somatic; Cytochrome complex; cyt c

Applications: Rat cytochrome C (CYCS) ELISA kit is used to quantify CYCS in serum, plasma, tissue homogenates, cell lysates, cell culture supernates, and other biological fluid samples of rat, providing data to support a wide range of studies.

Research Area: Metabolic pathway

Specification

Sample Type: Serum; Plasma; Tissue homogenates; Cell lysates; Cell culture supernates; Other biological fluids

Detection Range: 0.312-20 ng/mL

Sensitivity: 0.112 ng/mL

Precision (Intra-assay): CV<10%

Precision (Inter-assay): CV<12%

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at 2-8°C.

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