Mouse 11 beta hydroxysteroid dehydrogenase type 1 (11-beta-HSD1) ELISA kit (0.312-20 ng/mL)

Cat. No.: 0126WXX-366

Assay Type: Quantitative sandwich ELISA

Target Species: Mouse

Assay Target: 11-beta-HSD1

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Mouse 11 beta hydroxysteroid dehydrogenase type 1 (11-beta-HSD1) ELISA kit (0.312-20 ng/mL)

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Product Overview

Description: Mouse 11 beta hydroxysteroid dehydrogenase type 1 (11-beta-HSD1) ELISA kit (0.312-20 ng/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of 11-beta-HSD1 in mouse samples with a range of 0.312-20 ng/mL and a minimum detectable dose (sensitivity) of <0.115 ng/mL. The kit is highly sensitive and easy to use.

Assay Principle: This kit utilizes the sandwich enzyme immunoassay technique. The microtiter plate is pre-coated with an antibody specific to 11-beta-HSD1. Standards or samples are added to the wells along with a biotin-conjugated detection antibody. Subsequently, avidin-HRP is added and incubated. Upon the addition of TMB substrate, a color change occurs only in wells containing the target complex. The reaction is terminated with sulphuric acid and measured at 450 nm ± 10 nm. The concentration of 11-beta-HSD1 is determined by comparing the O.D. of the samples to the standard curve.

Background: 11β-HSD1 is a key redox enzyme localized to the endoplasmic reticulum. It reversibly catalyzes the conversion of cortisol to the inactive metabolite cortisone. Although this enzyme exhibits bidirectional activity in vitro experiments, within living cells, it primarily converts inactive cortisone to active cortisol, thereby increasing local glucocorticoid concentrations. Obesity and type 2 diabetes are associated with abnormalities in glucocorticoid metabolism. The link between 11β-HSD1 and obesity represents a critical and extensively studied area of research.

Synonyms: 11DH; 11-beta-HSD1; HDL; HSD11; HSD11B; HSD11L; SDR26C1; Corticosteroid 11-beta-dehydrogenase isozyme 1; Short chain dehydrogenase/reductase family 26C, member 1; 11-Beta-Hydroxysteroid Dehydrogenase Type 1; EC 1.1.1.146

EC NO.: 1.1.1.146

Applications: Mouse 11 beta hydroxysteroid dehydrogenase type 1 (11-beta-HSD1) ELISA kit (0.312-20 ng/mL) is used to quantify 11-beta-HSD1 in tissue homogenates, cell lysates, and other biological fluids of mouse, providing data to support research in a wide range of areas, including enzyme & kinase, metabolic pathway, endocrinology, cardiovascular biology, neuro science, hepatology, hormone metabolism, obesity, and others.

Research Area: Enzyme & kinase; Metabolic pathway; Endocrinology; Cardiovascular biology; Neuro science; Hepatology; Hormone metabolism; Obesity

Specification

Sample Type: Tissue homogenates; Cell lysates; Other biological fluids

Detection Range: 0.312-20 ng/mL

Sensitivity: <0.115 ng/mL

Precision (Intra-assay): CV<10%

Precision (Inter-assay): CV<12%

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at 2-8°C.

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