Porcine adiponectin (ADP) ELISA kit (62.5-4000 ng/mL)

Cat. No.: OB0525WXX-285

Assay Type: Quantitative sandwich ELISA

Target Species: Porcine

Assay Target: ADP

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Porcine adiponectin (ADP) ELISA kit (62.5-4000 ng/mL)

INQUIRY Datasheet MSDS

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Product Overview

Description: Porcine adiponectin (ADP) ELISA kit (62.5-4000 ng/mL) is a ready-to-use ELISA kit for analyzing porcine ADP levels with a detection range of 62.5-4000 ng/mL and a sensitivity of 37.5 ng/mL.

Assay Principle: The kit is based on the sandwich ELISA technique. The capture antibody is pre-coated in a 96-well plate. A biotin-conjugated antibody is used as the detection antibody. Standards, test samples, and biotin-conjugated detection antibodies are subsequently added to the wells and washed with buffer. HRP-streptavidin is added, and the unbound conjugate is washed away with washing solution. TMB substrate is used to visualize the HRP enzyme reaction. TMB produces a blue product catalyzed by HRP, which turns yellow upon addition of an acidic termination solution. The density of the yellow color is proportional to the amount of target sample captured in the plate. The concentration of the target can be calculated by reading the absorbance at 450 nm with a microplate reader.

Background: ADP is an important adipokine involved in the regulation of fat metabolism and insulin sensitivity, exhibiting antidiabetic, anti-atherosclerotic, and anti-inflammatory properties. It increases glucose utilization and fatty acid burning, stimulates AMPK phosphorylation and activation in liver and skeletal muscle, and may play a role in cell growth, angiogenesis, and tissue remodeling.

Synonyms: ADIPOQ; ACDC; APM1; Adipocyte C1q and collagen domain-containing protein; ACRP30; Adipose most abundant gene transcript 1 protein; apM-1; Gelatin-binding protein; Adiponectin

Applications: Porcine adiponectin (ADP) ELISA kit (62.5-4000 ng/mL) is designed for the in vitro quantitative analysis of ADP levels in porcine serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples.

Research Area: Hormone research; Lipid metabolism; Cell differentiation; Obesity

Specification

Sample Type: Serum; Plasma; Cell culture supernatant; Cell or tissue lysate; Other liquid samples

Detection Range: 62.5-4000 ng/mL

Sensitivity: 37.5 ng/mL

Precision (Intra-assay): CV<8%

Precision (Inter-assay): CV<10%

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at -20°C (microplate, standard) and 2-8°C (other reagents).

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