Bovine C-reactive protein (CRP) ELISA kit (39.063-2500 ng/mL)

Cat. No.: OB0625WXX-427

Assay Type: Quantitative sandwich ELISA

Target Species: Bovine

Assay Target: CRP

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Bovine C-reactive protein (CRP) ELISA kit (39.063-2500 ng/mL)

INQUIRY Datasheet MSDS

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Product Overview

Description: Bovine C-reactive protein (CRP) ELISA kit (39.063-2500 ng/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of CRP in bovine with a range of 39.063-2500 ng/mL and a specificity of 23.438 ng/mL.

Assay Principle: The anti-bovine CRP antibody is pre-coated onto a 96-well plate. Biotin-conjugated anti-bovine CRP antibody is then used as the detection antibody. Standards, assay samples, and biotin-conjugated detection antibody are then added to the wells and washed with buffer. HRP-streptavidin is added, and the unbound conjugate is washed away with washing solution. TMB substrate is used to observe the HRP enzymatic reaction. TMB produces a blue product catalyzed by HRP, which turns yellow upon addition of an acidic termination solution. The density of the yellow color is proportional to the amount of bovine CRP sample captured in the plate. The concentration of bovine CRP can be calculated by reading the absorbance at 450nm with a microplate reader.

Background: CRP is an acute-phase protein of hepatic origin, whose circulating concentrations rise in response to inflammation. It is considered a key plasma protein that can bind to Leptin, and its potential role in regulating leptin's function in obesity is crucial.

Synonyms: Pentraxin-related; pentraxin 1; C-RP; PTX1

Applications: Bovine C-reactive protein (CRP) ELISA kit (39.063-2500 ng/mL) is used to quantify CRP in serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples of bovine, providing data to support a wide range of studies.

Research Area: Inflammatory reaction; Obesity research

Specification

Sample Type: Serum; Plasma; Cell culture supernatant; Cell or tissue lysate; Other liquid samples

Detection Range: 39.063-2500 ng/mL

Sensitivity: 23.438 ng/mL

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at -20°C (microplate, standard) and 2-8°C (other reagents).

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