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Vitamin B12 test kit

Human 3 beta-hydroxysteroid dehydrogenase/delta 5-->4-isomerase type 1 (HSD3B1) ELISA kit (0.312-20 ng/mL)

Cat. No.: 0126WXX-379
Assay Type: Quantitative competitive ELISA
Target Species: Human
Assay Target: HSD3B1
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Human 3 beta-hydroxysteroid dehydrogenase/delta 5-->4-isomerase type 1 (HSD3B1) ELISA kit (0.312-20 ng/mL)
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Product Overview

Description: Human 3 beta-hydroxysteroid dehydrogenase/delta 5-->4-isomerase type 1 (HSD3B1) ELISA kit (0.312-20 ng/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of HSD3B1 in human samples with a range of 0.313-20 ng/mL and a minimum detectable dose (sensitivity) of 0.188 ng/mL. The kit is highly sensitive and easy to use.
Assay Principle: The ELISA analytical biochemical technique is based on HSD3B1 antibody-HSD3B1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect HSD3B1 antigen targets in samples.
Background: HSD3B1 is a bifunctional enzyme that plays a pivotal role in steroid hormone synthesis. It catalyzes key steps in multiple steroid biosynthesis pathways. Given its role in regulating metabolism and energy balance, it may serve as a potential regulator of obesity.
Synonyms: SDR11E1; Short chain dehydrogenase/reductase family 11E, Member 1; 3-beta-hydroxy-delta(5)-steroid dehydrogenase; Dihydrotestosterone oxidoreductase
Applications: Human 3 beta-hydroxysteroid dehydrogenase/delta 5-->4-isomerase type 1 (HSD3B1) ELISA kit (0.312-20 ng/mL) is used to quantify HSD3B1 in serum, plasma, cell culture supernatant, cell, tissue lysate, and other liquid samples of human, providing data to support research in a wide range of areas, including endocrinology, metabolism, obesity, and others.
Research Area: Endocrinology; Metabolism; Obesity

Specification

Sample Type: Serum; Plasma; Cell culture supernatant; Cell; Tissue lysate; Other liquid samples
Detection Range: 0.313-20 ng/mL
Sensitivity: 0.188 ng/mL
Precision (Intra-assay): CV<8%
Precision (Inter-assay): CV<10%
Cross-reactivity: No significant cross-reactivity or interference was observed.
Recovery: 0.94
Storage: Store at -20°C (microplate, standard) and 2-8°C (other reagents).
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