AdipoUpX™ Human WNT10B Lentiviral Particle (Overexpression)

Product Overview

Description: AdipoUpX™ Human WNT10B Lentiviral Particle (Overexpression) is professionally engineered for the long-lasting, stable overexpression of the human WNT10B gene. As a potent endogenous inhibitor of adipogenesis, its stable integration into the host genome is an ideal tool for researching the maintenance of lean phenotypes and bone-fat balance. The product is high-titer and ready-to-use, with all particles undergoing rigorous internal quality control validation (titer, sterility, and mycoplasma). Our customizable platform allows researchers to flexibly select from multiple promoters for precise targeted expression.

Production Cell Line: HEK293T

Promoter: Ubi; CMV; EF1a; Others

Product Availability: Produced Upon Order

Specification

Titer Test: qPCR

Insert Verification: PCR analysis was performed to verify the complete genomic sequence of all viral vector preparations.

Sterility Test: This product was certified sterile following successful microbial growth analysis.

Mycoplasma Test: The production quality control confirmed the absence of any mycoplasmal agents.

Other QC: This comprehensive process includes customized supplementary testing alongside in vitro and in vivo transduction assessments to elucidate transgene expression and functionality.

Storage: To maintain product integrity, immediate transfer of the vector to a -80 °C is essential following its receipt.

Stability: Continuous storage at -80°C ensures excellent long-term stability for 6-12 months. Once thawed, the product's working solution should be refrigerated at 4°C and used within 2-3 weeks.

Shipping Condition: Our lentivirus viral products are shipped using dry ice.

Handling Notes: Ensure all operations are performed within a biosafety cabinet; upon receiving the vector, immediately aliquot it into low-protein-binding tubes and store according to specified conditions, thus avoiding the titer loss caused by sensitivity to temperature fluctuations and freeze-thaw cycles.

Intended Use: This product is intended for research use only and is not for use in diagnosis or therapeutic applications.

Product Disclaimer: Given the diversity and complexity of experimental conditions, we do not guarantee product performance or experimental results in any specific application, despite our company's commitment to product quality and the rigorous internal QC inspections all products have undergone. Ultimate responsibility remains with the user for all operational processes, including storage, handling, and compliance with safety and biosafety protocols.

Target Profile

Gene Name: WNT10B

Full Name: Wnt family member 10B

Gene Symbol: SHFM6; STHAG8; WNT-12

Gene ID: 7480

RefSeq ID-1: NP_003385.2

RefSeq ID-2: NM_003394.3

Summary: WNT10B encodes a secreted signaling protein of the WNT family, which regulates cell fate determination and embryonic development. WNT10B signaling acts as a molecular switch controlling adipogenesis by inhibiting adipocyte differentiation. Dysregulation of WNT10B activity can alter adipose tissue development and contribute to obesity. The protein is highly conserved, sharing 96% amino acid identity with mouse Wnt10b, and the gene is clustered with WNT1 on chromosome 12q13.

AdipoUpX™ Human WNT10B Lentiviral Particle (Overexpression)

Product Overview

Specification

Target Profile

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