Product Overview
Description:
AdipoUpX™ Human ATF6 Lentiviral Particle (Overexpression) is a ready-to-use, high-titer vector particle professionally engineered for the stable overexpression of the human ATF6 gene. As a key sensor of endoplasmic reticulum stress, its stable expression in cell models allows for in-depth research into the UPR pathway and its link to insulin resistance. To ensure the highest research standards, every batch undergoes comprehensive quality control, including titer, sterility, and mycoplasma testing. The lentivirus characteristic of genome integration establishes reliable, long-lasting cell models for metabolic stress studies.
Production Cell Line:
HEK293T
Promoter:
Ubi; CMV; EF1a; Others
Product Availability:
Produced Upon Order
Specification
Titer Test:
qPCR
Insert Verification:
PCR analysis was performed to verify the complete genomic sequence of all viral vector preparations.
Sterility Test:
This product was certified sterile following successful microbial growth analysis.
Mycoplasma Test:
The production quality control confirmed the absence of any mycoplasmal agents.
Other QC:
This comprehensive process includes customized supplementary testing alongside in vitro and in vivo transduction assessments to elucidate transgene expression and functionality.
Storage:
To maintain product integrity, immediate transfer of the vector to a -80 °C is essential following its receipt.
Stability:
Continuous storage at -80°C ensures excellent long-term stability for 6-12 months. Once thawed, the product's working solution should be refrigerated at 4°C and used within 2-3 weeks.
Shipping Condition:
Our lentivirus viral products are shipped using dry ice.
Handling Notes:
Ensure all operations are performed within a biosafety cabinet; upon receiving the vector, immediately aliquot it into low-protein-binding tubes and store according to specified conditions, thus avoiding the titer loss caused by sensitivity to temperature fluctuations and freeze-thaw cycles.
Intended Use:
This product is intended for research use only and is not for use in diagnosis or therapeutic applications.
Product Disclaimer:
Given the diversity and complexity of experimental conditions, we do not guarantee product performance or experimental results in any specific application, despite our company's commitment to product quality and the rigorous internal QC inspections all products have undergone. Ultimate responsibility remains with the user for all operational processes, including storage, handling, and compliance with safety and biosafety protocols.
Target Profile
Gene Name:
ATF6
Full Name:
Activating transcription factor 6
Gene Symbol:
ACHM7; ATF6A; ATP6alpha
Summary:
ATF6 encodes a key transcription factor involved in the unfolded protein response (UPR) activated during endoplasmic reticulum (ER) stress. Uniquely, ATF6 is synthesized as an ER-resident transmembrane protein that functions as a stress sensor; upon ER stress–induced proteolytic cleavage, its cytosolic domain translocates to the nucleus, where it binds ER stress response elements (ERSEs) in the promoters of ER chaperone genes. Beyond its role in cellular stress adaptation, ATF6 has been implicated in metabolic homeostasis, as chronic ER stress in adipose tissue and liver contributes to obesity-associated insulin resistance. Genetic variation in ATF6 has shown population-dependent associations with diabetes and plasma lipid levels, linking ER stress signaling to obesity-related metabolic disorders. ATF6 has also been identified as a survival factor in quiescent squamous carcinoma cells and proposed as a therapeutic target in cystic fibrosis.
