AdipoUpX™ Human SIM1 Lentiviral Particle (Overexpression)

Product Overview

Description: AdipoUpX™ Human SIM1 Lentiviral Particle (Overexpression) is a high-titer system for the specific and stable expression of the human SIM1 gene. As a critical transcription factor for hypothalamic development, its stable overexpression enables researchers to establish reliable cell models for studying satiety signaling and appetite suppression. The lentivirus characteristic of genome integration facilitates long-term functional validation. Comprehensive quality control is performed on all products to ensure safety, efficacy, and the reproducibility of experimental results.

Production Cell Line: HEK293T

Promoter: Ubi; CMV; EF1a; Others

Product Availability: Produced Upon Order

Specification

Titer Test: qPCR

Insert Verification: PCR analysis was performed to verify the complete genomic sequence of all viral vector preparations.

Sterility Test: This product was certified sterile following successful microbial growth analysis.

Mycoplasma Test: The production quality control confirmed the absence of any mycoplasmal agents.

Other QC: This comprehensive process includes customized supplementary testing alongside in vitro and in vivo transduction assessments to elucidate transgene expression and functionality.

Storage: To maintain product integrity, immediate transfer of the vector to a -80 °C is essential following its receipt.

Stability: Continuous storage at -80°C ensures excellent long-term stability for 6-12 months. Once thawed, the product's working solution should be refrigerated at 4°C and used within 2-3 weeks.

Shipping Condition: Our lentivirus viral products are shipped using dry ice.

Handling Notes: Ensure all operations are performed within a biosafety cabinet; upon receiving the vector, immediately aliquot it into low-protein-binding tubes and store according to specified conditions, thus avoiding the titer loss caused by sensitivity to temperature fluctuations and freeze-thaw cycles.

Intended Use: This product is intended for research use only and is not for use in diagnosis or therapeutic applications.

Product Disclaimer: Given the diversity and complexity of experimental conditions, we do not guarantee product performance or experimental results in any specific application, despite our company's commitment to product quality and the rigorous internal QC inspections all products have undergone. Ultimate responsibility remains with the user for all operational processes, including storage, handling, and compliance with safety and biosafety protocols.

Target Profile

Gene Name: SIM1

Full Name: SIM bHLH transcription factor 1

Gene Symbol: bHLHe14

Gene ID: 6492

RefSeq ID-1: NP_001361698.1

RefSeq ID-2: NM_001374769.1

Summary: SIM1 is the human homolog of the Drosophila single-minded (sim) gene. Although its expression profile is narrow, notably detected in the fetal kidney, its evolutionary role suggests a crucial function in neurogenesis and brain development. The SIM family plays vital roles in developmental patterning. Consequently, SIM1 is proposed as a strong candidate gene involved in specific dysmorphic features, brain development abnormalities, and cognitive impairments often associated with syndromes like Down Syndrome.

AdipoUpX™ Human SIM1 Lentiviral Particle (Overexpression)

Product Overview

Specification

Target Profile

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