LipoKnoxa™ Human DGAT2 shRNA Lentiviral Particle (Silencing)

Sub Cat. No.	TargetSeq	Region	Inquiry
V0126XX589-1	ATGGGTGTCTGTGGGTTATTT	3' UTR	Inquiry
V0126XX589-2	GTTCTAGGTGGTGGCTAAATC	3' UTR	Inquiry
V0126XX589-3	CCGGGACACCATAGACTATTT	CDS	Inquiry
V0126XX589-4	Other		Inquiry

Product Overview

Description: LipoKnoxa™ Human DGAT2 shRNA Lentiviral Particle (Silencing) targets diacylglycerol O-acyltransferase 2, a major enzyme responsible for hepatic and adipose triglyceride synthesis. Our system provides two distinct expression strategies: high-potency U6 systems for maximal silencing and physiologically refined miR30-based shRNA for optimized processing and reduced off-target effects. To ensure the highest level of data reliability, every preparation undergoes comprehensive QC verification—covering functional titer, sequence identity, and mycoplasma testing—ensuring its effectiveness in lipid metabolism research.

Production Cell Line: HEK293T

Promoter: U6; CMV; EF1α; CAG; UBC

Product Availability: Produced Upon Order

Specification

Titer Test: qPCR

Insert Verification: Comprehensive sequencing and PCR analyses were performed to verify the accurate genomic sequence of all viral preparations.

Sterility Test: Confirmed sterile via rigorous microbial analysis; free of bacterial and fungal contamination.

Mycoplasma Test: Rigorous quality control testing has confirmed the absence of mycoplasma contamination in this viral preparation.

Other QC: Customized supplementary testing and in vitro/in vivo assessments are available to verify post-silencing gene expression and biological functionality, ensuring the viral preparations meet the specific potency requirements of your gene interference project.

Storage: Store at -80°C for long-term preservation. Immediate transfer upon delivery is required to prevent loss of viral activity.

Stability: Shelf life: 6–12 months at -80°C (extended stability up to 2 years). Post-thaw: Stable at 4°C for 2–3 weeks without significant degradation of functional titers.

Shipping Condition: Products are delivered on dry ice to maintain the cold chain. Upon arrival, please ensure the presence of dry ice and transfer the vials immediately to -80°C storage.

Handling Notes: Avoid repeated freeze-thaw cycles. Aliquot into low-protein-binding tubes immediately after receipt to maintain optimal activity. For safety and contamination control, all viral handling must be performed inside a biosafety cabinet.

Intended Use: This product is intended for research use only and is not for use in diagnosis or therapeutic applications.

Product Disclaimer: We ensure product integrity through rigorous QC, but we do not guarantee results in specific research contexts. Proper storage, handling, and total compliance with biosafety laws and safety protocols remain the sole responsibility of the user.

Target Profile

Gene Name: DGAT2

Full Name: Diacylglycerol O-acyltransferase 2

Gene Symbol: ARAT; HMFN1045; GS1999FULL

Gene ID: 84649

RefSeq ID-1: NP_001240820.1

RefSeq ID-2: NM_001253891.2

Summary: DGAT2 encodes an enzyme catalyzing the final step of triglyceride synthesis, directly controlling fat storage in adipocytes. Its activity influences energy storage efficiency and susceptibility to obesity. Multiple transcript variants exist due to alternative splicing.

LipoKnoxa™ Human DGAT2 shRNA Lentiviral Particle (Silencing)

Product Overview

Specification

Target Profile

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