AdipoUpX™ Human UCP1 Lentiviral Particle (Overexpression)

Product Overview

Description: AdipoUpX™ Human UCP1 Lentiviral Particle (Overexpression) is a powerful, high-titer gene delivery tool specifically designed for the stable overexpression of the human UCP1 gene. This product is a key asset for studying mitochondrial thermogenesis and energy expenditure in "beige" adipocytes. Genome integration enables the long-term validation of UCP1’s anti-obesity effects. All viral products undergo rigorous internal quality control to ensure reliability, and clients can flexibly customize promoters and labels to suit their specific research models.

Production Cell Line: HEK293T

Promoter: Ubi; CMV; EF1a; Others

Product Availability: Produced Upon Order

Specification

Titer Test: qPCR

Insert Verification: PCR analysis was performed to verify the complete genomic sequence of all viral vector preparations.

Sterility Test: This product was certified sterile following successful microbial growth analysis.

Mycoplasma Test: The production quality control confirmed the absence of any mycoplasmal agents.

Other QC: This comprehensive process includes customized supplementary testing alongside in vitro and in vivo transduction assessments to elucidate transgene expression and functionality.

Storage: To maintain product integrity, immediate transfer of the vector to a -80 °C is essential following its receipt.

Stability: Continuous storage at -80°C ensures excellent long-term stability for 6-12 months. Once thawed, the product's working solution should be refrigerated at 4°C and used within 2-3 weeks.

Shipping Condition: Our lentivirus viral products are shipped using dry ice.

Handling Notes: Ensure all operations are performed within a biosafety cabinet; upon receiving the vector, immediately aliquot it into low-protein-binding tubes and store according to specified conditions, thus avoiding the titer loss caused by sensitivity to temperature fluctuations and freeze-thaw cycles.

Intended Use: This product is intended for research use only and is not for use in diagnosis or therapeutic applications.

Product Disclaimer: Given the diversity and complexity of experimental conditions, we do not guarantee product performance or experimental results in any specific application, despite our company's commitment to product quality and the rigorous internal QC inspections all products have undergone. Ultimate responsibility remains with the user for all operational processes, including storage, handling, and compliance with safety and biosafety protocols.

Target Profile

Gene Name: UCP1

Full Name: Uncoupling protein 1

Gene Symbol: UCP; SLC25A7

Gene ID: 7350

RefSeq ID-1: NP_068605.1

RefSeq ID-2: NM_021833.5

Summary: UCP1 is the prototypical member of the mitochondrial uncoupling protein family, embedded in the inner mitochondrial membrane. Its function is unique and specialized: it mediates the "mitochondrial proton leak," a process that uncouples oxidative phosphorylation from ATP synthesis, allowing the potential energy of the proton gradient to be rapidly dissipated solely as heat. UCP1 expression is strictly restricted to brown adipose tissue (BAT), the specialized fat tissue dedicated to nonshivering thermogenesis. This protein is critical for regulating body temperature and is highly studied for its potential role in combating obesity by increasing energy expenditure.

AdipoUpX™ Human UCP1 Lentiviral Particle (Overexpression)

Product Overview

Specification

Target Profile

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