AdipoUpX™ Human ACACA Lentiviral Particle (Overexpression)

Product Overview

Description: AdipoUpX™ Human ACACA Lentiviral Particle (Overexpression) is a ready-to-use, high-titer vector particle designed for the efficient and stable overexpression of the human ACACA (ACC1) gene. It serves as a powerful tool for researching the rate-limiting step in fatty acid synthesis within the cytosol. Genome integration ensures the target gene integrates into the host cell genome, establishing reliable, stable cell lines for metabolic flux analysis. Every lot passes rigorous quality control for titer, sterility, and mycoplasma, ensuring the highest standards of experimental integrity.

Production Cell Line: HEK293T

Promoter: Ubi; CMV; EF1a; Others

Product Availability: Produced Upon Order

Specification

Titer Test: qPCR

Insert Verification: PCR analysis was performed to verify the complete genomic sequence of all viral vector preparations.

Sterility Test: This product was certified sterile following successful microbial growth analysis.

Mycoplasma Test: The production quality control confirmed the absence of any mycoplasmal agents.

Other QC: This comprehensive process includes customized supplementary testing alongside in vitro and in vivo transduction assessments to elucidate transgene expression and functionality.

Storage: To maintain product integrity, immediate transfer of the vector to a -80 °C is essential following its receipt.

Stability: Continuous storage at -80°C ensures excellent long-term stability for 6-12 months. Once thawed, the product's working solution should be refrigerated at 4°C and used within 2-3 weeks.

Shipping Condition: Our lentivirus viral products are shipped using dry ice.

Handling Notes: Ensure all operations are performed within a biosafety cabinet; upon receiving the vector, immediately aliquot it into low-protein-binding tubes and store according to specified conditions, thus avoiding the titer loss caused by sensitivity to temperature fluctuations and freeze-thaw cycles.

Intended Use: This product is intended for research use only and is not for use in diagnosis or therapeutic applications.

Product Disclaimer: Given the diversity and complexity of experimental conditions, we do not guarantee product performance or experimental results in any specific application, despite our company's commitment to product quality and the rigorous internal QC inspections all products have undergone. Ultimate responsibility remains with the user for all operational processes, including storage, handling, and compliance with safety and biosafety protocols.

Target Profile

Gene Name: ACACA

Full Name: Acetyl-CoA carboxylase alpha

Gene Symbol: ACC; ACAC; ACC1; ACCA; Acac1; hACC1; ACACAD; ACCalpha; ACACalpha

Gene ID: 31

RefSeq ID-1: NP_942131.1

RefSeq ID-2: NM_198834.3

Summary: ACACA encodes a multifunctional biotin-containing enzyme that catalyzes the rate-limiting step in fatty acid synthesis: the conversion of acetyl-CoA to malonyl-CoA. Highly enriched in adipose and hepatic tissues, the enzyme is under rigorous hormonal and allosteric control. Its activity levels directly determine the flux of carbon into lipid storage, making it a central node in the regulation of body fat accumulation.

AdipoUpX™ Human ACACA Lentiviral Particle (Overexpression)

Product Overview

Specification

Target Profile

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