AdipoUpX™ Human EIF2AK3 Lentiviral Particle (Overexpression)

Product Overview

Description: AdipoUpX™ Human EIF2AK3 Lentiviral Particle (Overexpression) is a high-titer system for the specific and stable expression of the human EIF2AK3 (PERK) gene. It serves as a vital tool for establishing stable cell models to investigate how the unfolded protein response (UPR) regulates beta-cell survival and insulin secretion. The lentivirus characteristic of genome integration facilitates long-term study of ER stress cascades. Comprehensive quality control is performed to ensure both product safety and the reproducibility of metabolic results.

Production Cell Line: HEK293T

Promoter: Ubi; CMV; EF1a; Others

Product Availability: Produced Upon Order

Specification

Titer Test: qPCR

Insert Verification: PCR analysis was performed to verify the complete genomic sequence of all viral vector preparations.

Sterility Test: This product was certified sterile following successful microbial growth analysis.

Mycoplasma Test: The production quality control confirmed the absence of any mycoplasmal agents.

Other QC: This comprehensive process includes customized supplementary testing alongside in vitro and in vivo transduction assessments to elucidate transgene expression and functionality.

Storage: To maintain product integrity, immediate transfer of the vector to a -80 °C is essential following its receipt.

Stability: Continuous storage at -80°C ensures excellent long-term stability for 6-12 months. Once thawed, the product's working solution should be refrigerated at 4°C and used within 2-3 weeks.

Shipping Condition: Our lentivirus viral products are shipped using dry ice.

Handling Notes: Ensure all operations are performed within a biosafety cabinet; upon receiving the vector, immediately aliquot it into low-protein-binding tubes and store according to specified conditions, thus avoiding the titer loss caused by sensitivity to temperature fluctuations and freeze-thaw cycles.

Intended Use: This product is intended for research use only and is not for use in diagnosis or therapeutic applications.

Product Disclaimer: Given the diversity and complexity of experimental conditions, we do not guarantee product performance or experimental results in any specific application, despite our company's commitment to product quality and the rigorous internal QC inspections all products have undergone. Ultimate responsibility remains with the user for all operational processes, including storage, handling, and compliance with safety and biosafety protocols.

Target Profile

Gene Name: EIF2AK3

Full Name: Eukaryotic translation initiation factor 2 alpha kinase 3

Gene Symbol: PEK; WRS; PERK

Gene ID: 9451

RefSeq ID-1: NP_004827.4

RefSeq ID-2: NM_004836.6

Summary: EIF2AK3 is a sentinel protein in the endoplasmic reticulum (ER) that monitors the health of protein folding. Under "ER stress"—often caused by nutrient overload—this protein halts global protein synthesis to prevent further damage. While protective, chronic activation of this pathway can lead to cell death, as seen in Wolcott-Rallison syndrome and type 2 diabetes.

AdipoUpX™ Human EIF2AK3 Lentiviral Particle (Overexpression)

Product Overview

Specification

Target Profile

Related Products