AdipoUpX™ Human ITGAM Lentiviral Particle (Overexpression)

Product Overview

Description: AdipoUpX™ Human ITGAM Lentiviral Particle (Overexpression) is professionally engineered for the efficient and stable expression of the human ITGAM (CD11b) gene. This integrin is a critical marker for researching macrophage infiltration in obese adipose tissue and the resulting chronic low-grade inflammation. The lentivirus characteristics facilitate the establishment of permanent cell models for high-fidelity functional validation. Every batch is verified through comprehensive quality control screening (titer, sterility, and mycoplasma) to guarantee experimental safety and data integrity.

Production Cell Line: HEK293T

Promoter: Ubi; CMV; EF1a; Others

Product Availability: Produced Upon Order

Specification

Titer Test: qPCR

Insert Verification: PCR analysis was performed to verify the complete genomic sequence of all viral vector preparations.

Sterility Test: This product was certified sterile following successful microbial growth analysis.

Mycoplasma Test: The production quality control confirmed the absence of any mycoplasmal agents.

Other QC: This comprehensive process includes customized supplementary testing alongside in vitro and in vivo transduction assessments to elucidate transgene expression and functionality.

Storage: To maintain product integrity, immediate transfer of the vector to a -80 °C is essential following its receipt.

Stability: Continuous storage at -80°C ensures excellent long-term stability for 6-12 months. Once thawed, the product's working solution should be refrigerated at 4°C and used within 2-3 weeks.

Shipping Condition: Our lentivirus viral products are shipped using dry ice.

Handling Notes: Ensure all operations are performed within a biosafety cabinet; upon receiving the vector, immediately aliquot it into low-protein-binding tubes and store according to specified conditions, thus avoiding the titer loss caused by sensitivity to temperature fluctuations and freeze-thaw cycles.

Intended Use: This product is intended for research use only and is not for use in diagnosis or therapeutic applications.

Product Disclaimer: Given the diversity and complexity of experimental conditions, we do not guarantee product performance or experimental results in any specific application, despite our company's commitment to product quality and the rigorous internal QC inspections all products have undergone. Ultimate responsibility remains with the user for all operational processes, including storage, handling, and compliance with safety and biosafety protocols.

Target Profile

Gene Name: ITGAM

Full Name: Integrin subunit alpha M

Gene Symbol: CR3A; MO1A; CD11B; MAC-1; MAC1A; SLEB6

Gene ID: 3684

RefSeq ID-1: NP_000623.2

RefSeq ID-2: NM_000632.4

Summary: ITGAM encodes the alpha M chain of the Mac-1 integrin, a critical receptor on the surface of white blood cells. This protein allows neutrophils and monocytes to adhere to the blood vessel walls and migrate toward sites of inflammation. In the context of metabolic health, ITGAM-positive cells are major players in the inflammatory infiltration of fat depots that occurs during weight gain.

AdipoUpX™ Human ITGAM Lentiviral Particle (Overexpression)

Product Overview

Specification

Target Profile

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