AdipoUpX™ Human AGRP Lentiviral Particle (Overexpression)

Product Overview

Description: AdipoUpX™ Human AGRP Lentiviral Particle (Overexpression) is a powerful, high-titer gene delivery tool specifically designed for the stable overexpression of the human AGRP gene. AGRP is a potent orexigenic peptide; this stable integration system is an ideal tool for studying the neurological triggers of hyperphagia and the antagonism of the MC4R pathway. All viral particle products undergo comprehensive quality control to ensure safety and efficacy. The system features a modular design, allowing researchers to customize promoters for specific neuronal models.

Production Cell Line: HEK293T

Promoter: Ubi; CMV; EF1a; Others

Product Availability: Produced Upon Order

Specification

Titer Test: qPCR

Insert Verification: PCR analysis was performed to verify the complete genomic sequence of all viral vector preparations.

Sterility Test: This product was certified sterile following successful microbial growth analysis.

Mycoplasma Test: The production quality control confirmed the absence of any mycoplasmal agents.

Other QC: This comprehensive process includes customized supplementary testing alongside in vitro and in vivo transduction assessments to elucidate transgene expression and functionality.

Storage: To maintain product integrity, immediate transfer of the vector to a -80 °C is essential following its receipt.

Stability: Continuous storage at -80°C ensures excellent long-term stability for 6-12 months. Once thawed, the product's working solution should be refrigerated at 4°C and used within 2-3 weeks.

Shipping Condition: Our lentivirus viral products are shipped using dry ice.

Handling Notes: Ensure all operations are performed within a biosafety cabinet; upon receiving the vector, immediately aliquot it into low-protein-binding tubes and store according to specified conditions, thus avoiding the titer loss caused by sensitivity to temperature fluctuations and freeze-thaw cycles.

Intended Use: This product is intended for research use only and is not for use in diagnosis or therapeutic applications.

Product Disclaimer: Given the diversity and complexity of experimental conditions, we do not guarantee product performance or experimental results in any specific application, despite our company's commitment to product quality and the rigorous internal QC inspections all products have undergone. Ultimate responsibility remains with the user for all operational processes, including storage, handling, and compliance with safety and biosafety protocols.

Target Profile

Gene Name: AGRP

Full Name: Agouti related neuropeptide

Gene Symbol: ART; AGRT; ASIP2

Gene ID: 181

RefSeq ID-1: NP_001129.1

RefSeq ID-2: NM_001138.2

Summary: AGRP encodes a potent neuropeptide that functions as a natural antagonist for the Melanocortin-3 and Melanocortin-4 receptors (MC3R and MC4R) in the hypothalamus. By blocking the binding of the satiety-promoting signal (alpha-MSH) to the MC4R, AGRP actively stimulates appetite and reduces energy expenditure. This powerful antagonism forms the key mechanism for hypothalamic control of feeding behavior and plays a central role in weight homeostasis. Gene mutations and copy number variations in AGRP have been clearly associated with late-onset obesity in humans.

AdipoUpX™ Human AGRP Lentiviral Particle (Overexpression)

Product Overview

Specification

Target Profile

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