AdipoUpX™ Human SIM1 Ad5 Particle (Overexpression)

Product Overview

Description: AdipoUpX™ Human SIM1 Ad5 Adenoviral Particle (Overexpression) is specifically designed for efficient transient overexpression of the human SIM1 gene. As a critical transcription factor for hypothalamic development, this tool accelerates functional validation of SIM1-mediated satiety pathways and energy intake regulation. High customization via reporter genes and rigorous quality control (titer and sterility) are integrated features of this powerful research tool.

Production Cell Line: HEK293

Viral Backbone: Adenovirus type5 (dE1/E3)

Promoter: CMV

Product Availability: Produced Upon Order

Specification

Titer Test: qPCR

Insert Verification: All viral preparations have been validated via PCR to confirm the integrity of their vector genomes.

Sterility Test: This product was confirmed to be sterile upon testing.

Mycoplasma Test: This product has passed quality control, confirming that it is free of mycoplasma contamination.

Other QC: We perform customized supplementary testing and in vitro/in vivo transduction assessments based on specific project requirements to analyze the expression levels and biological functions of transgenes.

Storage: Upon receipt of viral preparations, they must be immediately transferred to a -80°C environment for long-term storage.

Stability: The product guarantees excellent stability for 6-12 months when stored continuously at -80°C (and in some cases, up to 2 years). Upon thawing, the working solution is refrigerated at 4°C for up to 2-3 weeks without significant loss of biological activity.

Shipping Condition: Our adenovirus viral products are shipped using dry ice.

Handling Notes: Viral particles are highly sensitive to temperature fluctuations and freeze-thaw cycles. To maintain titer and avoid repeated freeze-thaw cycles, it is recommended to aliquot the vector immediately upon receipt into low-protein-binding tubes and store according to specified conditions. Ensure all operations are performed within a biosafety cabinet.

Intended Use: This product is intended for research use only and is not for use in diagnosis or therapeutic applications.

Product Disclaimer: Our company is committed to product quality, and all products have undergone rigorous internal QC inspections. Given the diversity and complexity of experimental conditions, we do not guarantee product performance or experimental results in any specific application. Users bear ultimate responsibility for product storage, handling, and compliance with all safety protocols, laws, regulations, and biosafety requirements throughout all operational processes.

Target Profile

Gene Name: SIM1

Full Name: SIM bHLH transcription factor 1

Gene Symbol: bHLHe14

Gene ID: 6492

RefSeq ID-1: NP_001361698.1

RefSeq ID-2: NM_001374769.1

Summary: SIM1 is one of two human homologs of the key Drosophila developmental gene, single-minded (sim). While its detectable expression is highly restricted in adult tissues, its role is proposed to be fundamental during neurogenesis and embryonic development. The SIM family's developmental importance suggests that human SIM1 is a critical factor in regulating brain architecture and potentially predisposing individuals to specific dysmorphic facial and skull characteristics and cognitive disability, often within the context of Down syndrome features.

AdipoUpX™ Human SIM1 Ad5 Particle (Overexpression)

Product Overview

Specification

Target Profile

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