AdipoUpX™ Human PRKAA1 Lentiviral Particle (Overexpression)

Product Overview

Description: AdipoUpX™ Human PRKAA1 Lentiviral Particle (Overexpression) is professionally engineered for the long-lasting, stable overexpression of the human PRKAA1 (AMPK alpha 1) gene. This ubiquitously expressed AMPK subunit is a key target for studying systemic metabolic health and cellular energy sensing under nutrient stress. Integrating into the host genome, it allows for the creation of stable cell lines to investigate AMPK-dependent phosphorylation cascades. The product is ready-to-use and high-titer, with all particles undergoing rigorous internal quality control validation (titer, sterility, and mycoplasma) to ensure the highest experimental standards.

Production Cell Line: HEK293T

Promoter: Ubi; CMV; EF1a; Others

Product Availability: Produced Upon Order

Specification

Titer Test: qPCR

Insert Verification: PCR analysis was performed to verify the complete genomic sequence of all viral vector preparations.

Sterility Test: This product was certified sterile following successful microbial growth analysis.

Mycoplasma Test: The production quality control confirmed the absence of any mycoplasmal agents.

Other QC: This comprehensive process includes customized supplementary testing alongside in vitro and in vivo transduction assessments to elucidate transgene expression and functionality.

Storage: To maintain product integrity, immediate transfer of the vector to a -80 °C is essential following its receipt.

Stability: Continuous storage at -80°C ensures excellent long-term stability for 6-12 months. Once thawed, the product's working solution should be refrigerated at 4°C and used within 2-3 weeks.

Shipping Condition: Our lentivirus viral products are shipped using dry ice.

Handling Notes: Ensure all operations are performed within a biosafety cabinet; upon receiving the vector, immediately aliquot it into low-protein-binding tubes and store according to specified conditions, thus avoiding the titer loss caused by sensitivity to temperature fluctuations and freeze-thaw cycles.

Intended Use: This product is intended for research use only and is not for use in diagnosis or therapeutic applications.

Product Disclaimer: Given the diversity and complexity of experimental conditions, we do not guarantee product performance or experimental results in any specific application, despite our company's commitment to product quality and the rigorous internal QC inspections all products have undergone. Ultimate responsibility remains with the user for all operational processes, including storage, handling, and compliance with safety and biosafety protocols.

Target Profile

Gene Name: PRKAA1

Full Name: Protein kinase AMP-activated catalytic subunit alpha 1

Gene Symbol: AMPK; AMPKa1; AMPK alpha 1

Gene ID: 5562

RefSeq ID-1: NP_006242.5

RefSeq ID-2: NM_006251.5

Summary: PRKAA1 encodes the alpha-1 catalytic subunit of the AMPK heterotrimer. Acting as a ubiquitous energy sensor, it is triggered by an increase in the cellular AMP/ATP ratio. By switching off ATP-consuming synthetic pathways and activating ATP-producing catabolic pathways, PRKAA1 protects cells from energy exhaustion during metabolic stress.

AdipoUpX™ Human PRKAA1 Lentiviral Particle (Overexpression)

Product Overview

Specification

Target Profile

Related Products