AdipoUpX™ Human NR0B2 Lentiviral Particle (Overexpression)

Product Overview

Description: AdipoUpX™ Human NR0B2 Lentiviral Particle (Overexpression) is professionally engineered for the efficient and stable expression of the human NR0B2 (SHP) gene. As a corepressor of nuclear receptors, its stable overexpression facilitates in-depth research into the regulation of bile acid synthesis and hepatic lipid metabolism. The lentivirus characteristic of genome integration establishes reliable, permanent cell models for long-term functional studies. To ensure the highest standards, all particles are screened through comprehensive quality control, including titer and mycoplasma detection.

Production Cell Line: HEK293T

Promoter: Ubi; CMV; EF1a; Others

Product Availability: Produced Upon Order

Specification

Titer Test: qPCR

Insert Verification: PCR analysis was performed to verify the complete genomic sequence of all viral vector preparations.

Sterility Test: This product was certified sterile following successful microbial growth analysis.

Mycoplasma Test: The production quality control confirmed the absence of any mycoplasmal agents.

Other QC: This comprehensive process includes customized supplementary testing alongside in vitro and in vivo transduction assessments to elucidate transgene expression and functionality.

Storage: To maintain product integrity, immediate transfer of the vector to a -80 °C is essential following its receipt.

Stability: Continuous storage at -80°C ensures excellent long-term stability for 6-12 months. Once thawed, the product's working solution should be refrigerated at 4°C and used within 2-3 weeks.

Shipping Condition: Our lentivirus viral products are shipped using dry ice.

Handling Notes: Ensure all operations are performed within a biosafety cabinet; upon receiving the vector, immediately aliquot it into low-protein-binding tubes and store according to specified conditions, thus avoiding the titer loss caused by sensitivity to temperature fluctuations and freeze-thaw cycles.

Intended Use: This product is intended for research use only and is not for use in diagnosis or therapeutic applications.

Product Disclaimer: Given the diversity and complexity of experimental conditions, we do not guarantee product performance or experimental results in any specific application, despite our company's commitment to product quality and the rigorous internal QC inspections all products have undergone. Ultimate responsibility remains with the user for all operational processes, including storage, handling, and compliance with safety and biosafety protocols.

Target Profile

Gene Name: NR0B2

Full Name: Nuclear receptor subfamily 0 group B member 2

Gene Symbol: SHP; SHP1

Gene ID: 8431

RefSeq ID-1: NP_068804.1

RefSeq ID-2: NM_021969.3

Summary: The protein encoded by NR0B2 is an atypical orphan nuclear receptor containing a putative ligand-binding domain but lacking a conventional DNA-binding domain. As a member of the nuclear hormone receptor family, it can interact with retinoid, thyroid, and estrogen receptors, repressing their ligand-dependent transcriptional activity through coactivator competition and direct transcriptional repression. Studies indicate that NR0B2 may influence metabolic regulation and energy homeostasis, and disruptions in its function have been linked to obesity, in addition to its roles in hormone signaling pathways.

AdipoUpX™ Human NR0B2 Lentiviral Particle (Overexpression)

Product Overview

Specification

Target Profile

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