AdipoUpX™ Human PRKAA2 Lentiviral Particle (Overexpression)

Product Overview

Description: AdipoUpX™ Human PRKAA2 Lentiviral Particle (Overexpression) is professionally engineered for the long-lasting, stable overexpression of the human PRKAA2 (AMPK alpha 2) gene. This catalytic subunit of AMPK is essential for researching cellular energy sensing and the activation of fatty acid oxidation. The product is ready-to-use and high-titer, with genome integration facilitating the creation of permanent cell models for functional validation. Comprehensive quality control, including titer and mycoplasma testing, is performed on all viral particles to ensure both product safety and experimental reproducibility.

Production Cell Line: HEK293T

Promoter: Ubi; CMV; EF1a; Others

Product Availability: Produced Upon Order

Specification

Titer Test: qPCR

Insert Verification: PCR analysis was performed to verify the complete genomic sequence of all viral vector preparations.

Sterility Test: This product was certified sterile following successful microbial growth analysis.

Mycoplasma Test: The production quality control confirmed the absence of any mycoplasmal agents.

Other QC: This comprehensive process includes customized supplementary testing alongside in vitro and in vivo transduction assessments to elucidate transgene expression and functionality.

Storage: To maintain product integrity, immediate transfer of the vector to a -80 °C is essential following its receipt.

Stability: Continuous storage at -80°C ensures excellent long-term stability for 6-12 months. Once thawed, the product's working solution should be refrigerated at 4°C and used within 2-3 weeks.

Shipping Condition: Our lentivirus viral products are shipped using dry ice.

Handling Notes: Ensure all operations are performed within a biosafety cabinet; upon receiving the vector, immediately aliquot it into low-protein-binding tubes and store according to specified conditions, thus avoiding the titer loss caused by sensitivity to temperature fluctuations and freeze-thaw cycles.

Intended Use: This product is intended for research use only and is not for use in diagnosis or therapeutic applications.

Product Disclaimer: Given the diversity and complexity of experimental conditions, we do not guarantee product performance or experimental results in any specific application, despite our company's commitment to product quality and the rigorous internal QC inspections all products have undergone. Ultimate responsibility remains with the user for all operational processes, including storage, handling, and compliance with safety and biosafety protocols.

Target Profile

Gene Name: PRKAA2

Full Name: Protein kinase AMP-activated catalytic subunit alpha 2

Gene Symbol: AMPK; AMPK2; PRKAA; AMPKa2

Gene ID: 5563

RefSeq ID-1: NP_006243.2

RefSeq ID-2: NM_006252.3

Summary: PRKAA2 encodes the alpha-2 catalytic subunit of AMP-activated protein kinase (AMPK), the cell's primary energy sensor. Activated by metabolic stress and high AMP levels, this subunit phosphorylates key enzymes like ACC and HMGCR to inhibit the synthesis of fatty acids and cholesterol. It is essential for maintaining whole-body insulin sensitivity and myocardial energy balance during ischemic events.

AdipoUpX™ Human PRKAA2 Lentiviral Particle (Overexpression)

Product Overview

Specification

Target Profile

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