AdipoUpX™ Human ABCA1 Lentiviral Particle (Overexpression)

Product Overview

Description: AdipoUpX™ Human ABCA1 Lentiviral Particle (Overexpression) is a powerful, high-titer gene delivery tool specifically designed for the stable overexpression of the human ABCA1 gene. The integration into the host genome facilitates the creation of stable cell lines for investigating cholesterol efflux and HDL biogenesis. This product accelerates progress in metabolic research by enabling rapid validation of lipid transport mechanisms. All viral particle products undergo rigorous internal quality control to ensure safety, and functional components like promoters and markers are highly customizable to meet diverse research needs.

Production Cell Line: HEK293T

Promoter: Ubi; CMV; EF1a; Others

Product Availability: Produced Upon Order

Specification

Titer Test: qPCR

Insert Verification: PCR analysis was performed to verify the complete genomic sequence of all viral vector preparations.

Sterility Test: This product was certified sterile following successful microbial growth analysis.

Mycoplasma Test: The production quality control confirmed the absence of any mycoplasmal agents.

Other QC: This comprehensive process includes customized supplementary testing alongside in vitro and in vivo transduction assessments to elucidate transgene expression and functionality.

Storage: To maintain product integrity, immediate transfer of the vector to a -80 °C is essential following its receipt.

Stability: Continuous storage at -80°C ensures excellent long-term stability for 6-12 months. Once thawed, the product's working solution should be refrigerated at 4°C and used within 2-3 weeks.

Shipping Condition: Our lentivirus viral products are shipped using dry ice.

Handling Notes: Ensure all operations are performed within a biosafety cabinet; upon receiving the vector, immediately aliquot it into low-protein-binding tubes and store according to specified conditions, thus avoiding the titer loss caused by sensitivity to temperature fluctuations and freeze-thaw cycles.

Intended Use: This product is intended for research use only and is not for use in diagnosis or therapeutic applications.

Product Disclaimer: Given the diversity and complexity of experimental conditions, we do not guarantee product performance or experimental results in any specific application, despite our company's commitment to product quality and the rigorous internal QC inspections all products have undergone. Ultimate responsibility remains with the user for all operational processes, including storage, handling, and compliance with safety and biosafety protocols.

Target Profile

Gene Name: ABCA1

Full Name: ATP binding cassette subfamily A member 1

Gene Symbol: TGD; ABC1; CERP; ABC-1; HDLDT1; HPALP1; HDLCQTL13

Gene ID: 19

RefSeq ID-1: NP_005493.2

RefSeq ID-2: NM_005502.3

Summary: ABCA1 is a critical membrane transporter that serves as a cholesterol efflux pump. It is the rate-limiting factor in the removal of cellular cholesterol and phospholipids, facilitating their transfer to apolipoprotein A-I to form High-Density Lipoprotein (HDL). Genetic dysfunction in ABCA1 leads to Tangier disease and familial HDL deficiency, highlighting its role in protecting cells from lipid overload and maintaining systemic lipid homeostasis in the context of adiposity.

AdipoUpX™ Human ABCA1 Lentiviral Particle (Overexpression)

Product Overview

Specification

Target Profile

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