AdipoUpX™ Human MAGEL2 Lentiviral Particle (Overexpression)

Product Overview

Description: AdipoUpX™ Human MAGEL2 Lentiviral Particle (Overexpression) is professionally engineered for the efficient and long-lasting overexpression of the human MAGEL2 gene. It is a reliable asset for studying the molecular pathogenesis of Prader-Willi and Schaaf-Yang syndromes, characterized by hyperphagia and obesity. Genome integration ensures the target gene establishes a permanent presence for longitudinal functional studies. Comprehensive quality control, including titer and mycoplasma testing, is performed on all viral products to ensure safety and data integrity.

Production Cell Line: HEK293T

Promoter: Ubi; CMV; EF1a; Others

Product Availability: Produced Upon Order

Specification

Titer Test: qPCR

Insert Verification: PCR analysis was performed to verify the complete genomic sequence of all viral vector preparations.

Sterility Test: This product was certified sterile following successful microbial growth analysis.

Mycoplasma Test: The production quality control confirmed the absence of any mycoplasmal agents.

Other QC: This comprehensive process includes customized supplementary testing alongside in vitro and in vivo transduction assessments to elucidate transgene expression and functionality.

Storage: To maintain product integrity, immediate transfer of the vector to a -80 °C is essential following its receipt.

Stability: Continuous storage at -80°C ensures excellent long-term stability for 6-12 months. Once thawed, the product's working solution should be refrigerated at 4°C and used within 2-3 weeks.

Shipping Condition: Our lentivirus viral products are shipped using dry ice.

Handling Notes: Ensure all operations are performed within a biosafety cabinet; upon receiving the vector, immediately aliquot it into low-protein-binding tubes and store according to specified conditions, thus avoiding the titer loss caused by sensitivity to temperature fluctuations and freeze-thaw cycles.

Intended Use: This product is intended for research use only and is not for use in diagnosis or therapeutic applications.

Product Disclaimer: Given the diversity and complexity of experimental conditions, we do not guarantee product performance or experimental results in any specific application, despite our company's commitment to product quality and the rigorous internal QC inspections all products have undergone. Ultimate responsibility remains with the user for all operational processes, including storage, handling, and compliance with safety and biosafety protocols.

Target Profile

Gene Name: MAGEL2

Full Name: MAGE family member L2

Gene Symbol: PWLS; nM15; NDNL1; SHFYNG

Gene ID: 54551

RefSeq ID-1: NP_061939.3

RefSeq ID-2: NM_019066.5

Summary: MAGEL2 is located within the chromosomal region associated with Prader-Willi syndrome (PWS) (15q11-q13). PWS is caused by the loss of expression of paternally inherited imprinted genes in this region, leading to a phenotype marked by neonatal hypotonia, developmental delay, and childhood-onset morbid obesity. MAGEL2 is structurally similar to Necdin (NDN), another gene implicated in PWS etiology, and is also paternally imprinted. Its localization and structure strongly suggest that MAGEL2 plays a necessary role in the development of the PWS phenotype, particularly the neuroendocrine dysfunction leading to hyperphagia and obesity.

AdipoUpX™ Human MAGEL2 Lentiviral Particle (Overexpression)

Product Overview

Specification

Target Profile

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