AdipoUpX™ Human OTOP1 Lentiviral Particle (Overexpression)

Product Overview

Description: AdipoUpX™ Human OTOP1 Lentiviral Particle (Overexpression) is professionally engineered for the long-lasting, stable overexpression of the human OTOP1 gene. This proton channel is essential for investigating intracellular pH regulation and its emerging role in the thermogenic response of brown adipose tissue. Integrating into the host genome, it allows for the creation of stable cell lines for high-fidelity functional validation. The product is ready-to-use and high-titer, and all particles undergo comprehensive quality control to ensure experimental safety and repeatability.

Production Cell Line: HEK293T

Promoter: Ubi; CMV; EF1a; Others

Product Availability: Produced Upon Order

Specification

Titer Test: qPCR

Insert Verification: PCR analysis was performed to verify the complete genomic sequence of all viral vector preparations.

Sterility Test: This product was certified sterile following successful microbial growth analysis.

Mycoplasma Test: The production quality control confirmed the absence of any mycoplasmal agents.

Other QC: This comprehensive process includes customized supplementary testing alongside in vitro and in vivo transduction assessments to elucidate transgene expression and functionality.

Storage: To maintain product integrity, immediate transfer of the vector to a -80 °C is essential following its receipt.

Stability: Continuous storage at -80°C ensures excellent long-term stability for 6-12 months. Once thawed, the product's working solution should be refrigerated at 4°C and used within 2-3 weeks.

Shipping Condition: Our lentivirus viral products are shipped using dry ice.

Handling Notes: Ensure all operations are performed within a biosafety cabinet; upon receiving the vector, immediately aliquot it into low-protein-binding tubes and store according to specified conditions, thus avoiding the titer loss caused by sensitivity to temperature fluctuations and freeze-thaw cycles.

Intended Use: This product is intended for research use only and is not for use in diagnosis or therapeutic applications.

Product Disclaimer: Given the diversity and complexity of experimental conditions, we do not guarantee product performance or experimental results in any specific application, despite our company's commitment to product quality and the rigorous internal QC inspections all products have undergone. Ultimate responsibility remains with the user for all operational processes, including storage, handling, and compliance with safety and biosafety protocols.

Target Profile

Gene Name: OTOP1

Full Name: Otopetrin 1

Gene ID: 133060

RefSeq ID-1: NP_819056.1

RefSeq ID-2: NM_177998.3

Summary: Originally discovered for its role in maintaining balance through the formation of inner ear minerals, OTOP1 has a surprising secondary function in adipose tissue. It acts as a proton channel that attenuates the inflammation induced by obesity. In fat cells, OTOP1 is part of a counter-inflammatory pathway that helps the body adapt to the metabolic stress of excess weight.

AdipoUpX™ Human OTOP1 Lentiviral Particle (Overexpression)

Product Overview

Specification

Target Profile

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