LipoKnoxa™ Human POMC shRNA Lentiviral Particle (Silencing)

Cat. No.: V0126XX424

Species: Human

Target Gene: POMC

Vector System: Lentiviral

Modulation Type: Silencing (shRNA)

LipoKnoxa™ Human POMC shRNA Lentiviral Particle (Silencing)

INQUIRY Datasheet MSDS

Sub Cat. No.	TargetSeq	Region	Inquiry
V0126XX424-1	ACTCCCATGTTCCCGGGAAAT	CDS	Inquiry
V0126XX424-2	CCAGTGAAGGTGTACCCTAAC	CDS	Inquiry
V0126XX424-3	CATCATCAAGAACGCCTACAA	CDS	Inquiry
V0126XX424-4	Other		Inquiry

Product Overview

Description: LipoKnoxa™ Human POMC shRNA Lentiviral Particle (Silencing) targets pro-opiomelanocortin, the precursor protein critical for anorexigenic signaling. By integrating either U6 or miR30-based shRNA into a high-titer lentiviral vector, this product ensures long-term gene silencing in both dividing and non-dividing neuronal populations. Each preparation is backed by comprehensive QC documentation, covering functional titer, sterility, and mycoplasma testing, supporting flexible selection of reporter genes for real-time monitoring of gene knockdown.

Production Cell Line: HEK293T

Promoter: U6; CMV; EF1α; CAG; UBC

Product Availability: Produced Upon Order

Specification

Titer Test: qPCR

Insert Verification: Comprehensive sequencing and PCR analyses were performed to verify the accurate genomic sequence of all viral preparations.

Sterility Test: Confirmed sterile via rigorous microbial analysis; free of bacterial and fungal contamination.

Mycoplasma Test: Rigorous quality control testing has confirmed the absence of mycoplasma contamination in this viral preparation.

Other QC: Customized supplementary testing and in vitro/in vivo assessments are available to verify post-silencing gene expression and biological functionality, ensuring the viral preparations meet the specific potency requirements of your gene interference project.

Storage: Store at -80°C for long-term preservation. Immediate transfer upon delivery is required to prevent loss of viral activity.

Stability: Shelf life: 6–12 months at -80°C (extended stability up to 2 years). Post-thaw: Stable at 4°C for 2–3 weeks without significant degradation of functional titers.

Shipping Condition: Products are delivered on dry ice to maintain the cold chain. Upon arrival, please ensure the presence of dry ice and transfer the vials immediately to -80°C storage.

Handling Notes: Avoid repeated freeze-thaw cycles. Aliquot into low-protein-binding tubes immediately after receipt to maintain optimal activity. For safety and contamination control, all viral handling must be performed inside a biosafety cabinet.

Intended Use: This product is intended for research use only and is not for use in diagnosis or therapeutic applications.

Product Disclaimer: We ensure product integrity through rigorous QC, but we do not guarantee results in specific research contexts. Proper storage, handling, and total compliance with biosafety laws and safety protocols remain the sole responsibility of the user.

Target Profile

Gene Name: POMC

Full Name: Pro-opiomelanocortin

Gene Symbol: LPH; MSH; NPP; POC; ACTH; CLIP; OBAIRH

Gene ID: 5443

RefSeq ID-1: NP_000930.1

RefSeq ID-2: NM_000939.4

Summary: The protein encoded by the POMC gene is one of the most complex peptide hormone precursors in the body. This precursor protein undergoes tissue-specific, intricate post-translational processing to yield up to ten bioactive peptides, including potent appetite suppressants (α-MSH), endorphins, adipokines, and ACTH. Differential processing of POMC peptides in the pituitary and hypothalamus confers their critical roles in energy homeostasis, adrenal function, and pain regulation. Furthermore, its mutations are associated with early-onset obesity, adrenal insufficiency, and distinctive hair pigmentation changes, reflecting its broad-spectrum effects across endocrine and neural systems.

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