Rabbit insulin like growth factor binding protein 2 (IGFBP-2) ELISA kit (0.156-10 ng/mL)

Cat. No.: 0126WXX-488

Assay Type: Quantitative sandwich ELISA

Target Species: Rabbit

Assay Target: IGFBP-2

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Rabbit insulin like growth factor binding protein 2 (IGFBP-2) ELISA kit (0.156-10 ng/mL)

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Product Overview

Description: Rabbit insulin like growth factor binding protein 2 (IGFBP-2) ELISA kit (0.156-10 ng/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of IGFBP-2 in rabbit samples with a range of 0.156-10 ng/mL and a minimum detectable dose (sensitivity) of 0.05 ng/mL. The kit is highly sensitive and easy to use.

Assay Principle: This experiment utilizes a double-sandwich ELISA technique. The microplate is pre-coated with a rabbit IGF-1 monoclonal antibody, and the detection antibody is a biotin-labeled polyclonal antibody. Samples and the detection antibody are added to the wells and washed with PBS or TBS. Subsequently, avidin-peroxidase conjugates and TMB substrate are added. The TMB turns blue under peroxidase catalysis and yellow upon the addition of acid. The color depth is positively correlated with the concentration of the target analyte in the samples.

Background: IGFBP-2 is a multifunctional molecule that plays a crucial role in human growth, metabolism, and cellular processes by regulating the biological activity of insulin-like growth factors (IGFs). Primarily through binding to IGF proteins, IGFBP-2 prolongs their half-life in the body and modulates their interactions with cell surface receptors, thereby inhibiting or enhancing the pro-growth effects of IGFs. The role of IGFBP-2 in obesity has garnered increasing attention. Its reduced levels are closely associated with obesity, dyslipidemia, and insulin resistance.

Synonyms: IBP-2; IGF-binding protein 2; IGFBP2; BP2, IBP2; Insulin-Like Growth Factor Binding Protein 2, 36kDa; Insulin-Like Growth Factor-Binding Protein 2

Applications: Rabbit insulin like growth factor binding protein 2 (IGFBP-2) ELISA kit (0.156-10 ng/mL) is used to quantify IGFBP-2 in rabbit serum, plasma, cell culture supernatant, and tissues of rabbits, providing data to support research in a wide range of areas, including immunity, endocrinology, signal transduction, obesity, and others.

Research Area: Immunity; Endocrinology; Signal transduction; Obesity

Specification

Sample Type: Rabbit serum; Plasma; Cell culture supernatant; Organizations

Detection Range: 0.156-10 ng/mL

Sensitivity: 0.05 ng/mL

Precision (Intra-assay): CV≤8%

Precision (Inter-assay): CV≤12%

Cross-reactivity: No significant cross-reactivity or interference was observed.

Recovery: 70-110%

Storage: Store at 2-8°C.

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