Porcine 3 beta-hydroxysteroid dehydrogenase/delta 5-->4-isomerase type 1 (HSD3B1) ELISA kit (0.312-20 ng/mL)

Cat. No.: 0126WXX-381

Assay Type: Quantitative sandwich ELISA

Target Species: Porcine

Assay Target: HSD3B1

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Porcine 3 beta-hydroxysteroid dehydrogenase/delta 5-->4-isomerase type 1 (HSD3B1) ELISA kit (0.312-20 ng/mL)

INQUIRY Datasheet MSDS

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Product Overview

Description: Porcine 3 beta-hydroxysteroid dehydrogenase/delta 5-->4-isomerase type 1 (HSD3B1) ELISA kit (0.312-20 ng/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of HSD3B1 in porcine samples with a range of 0.312-20 ng/mL and a minimum detectable dose (sensitivity) of 0.06 ng/mL. The kit is highly sensitive and easy to use.

Assay Principle: This assay employs a double-sandwich ELISA technique. The microplate is pre-coated with a porcine HSD3B1 monoclonal antibody, while the detection antibody is a biotin-labeled polyclonal antibody. Samples and the biotin-labeled antibody are added to the wells and subsequently washed with PBS or TBS. Next, avidin-peroxidase conjugates are added. After a thorough wash, the TMB substrate is introduced for color development. The solution turns blue under peroxidase catalysis and finally yellow upon the addition of acid. The color intensity is positively correlated with the concentration of HSD3B1 in the samples.

Background: HSD3B1 is a bifunctional enzyme that plays a pivotal role in steroid hormone synthesis. It catalyzes key steps in multiple steroid biosynthesis pathways. Given its role in regulating metabolism and energy balance, it may serve as a potential regulator of obesity.

Synonyms: SDR11E1; Short chain dehydrogenase/reductase family 11E, Member 1; 3-beta-hydroxy-delta(5)-steroid dehydrogenase; Dihydrotestosterone oxidoreductase

Applications: Porcine 3 beta-hydroxysteroid dehydrogenase/delta 5-->4-isomerase type 1 (HSD3B1) ELISA kit (0.312-20 ng/mL) is used to quantify HSD3B1 in porcine serum, plasma, cell culture supernatant, and tissues of porcine, providing data to support research in a wide range of areas, including endocrinology, metabolism, obesity, and others.

Research Area: Endocrinology; Metabolism; Obesity