Chicken C-reactive protein (CRP) ELISA kit (78.125-5000 pg/mL)

Cat. No.: OB0625WXX-436

Assay Type: Quantitative sandwich ELISA

Target Species: Chicken

Assay Target: CRP

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Chicken C-reactive protein (CRP) ELISA kit (78.125-5000 pg/mL)

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Product Overview

Description: Chicken C-reactive protein (CRP) ELISA kit (78.125-5000 pg/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of CRP in chicken with a range of 78.125-5000 pg/mL and a specificity of 46.875 pg/mL.

Assay Principle: The anti-CRP antibody is pre-coated onto a 96-well plate. A biotin-conjugated anti-CRP antibody is used as the detection antibody. Standards, test samples, and biotin-conjugated detection antibody are subsequently added to the wells and washed with buffer. HRP-Streptavidin is added, and the unbound conjugate is washed away with washing solution. TMB substrate is used to visualize the HRP enzyme reaction. TMB produces a blue product catalyzed by HRP, which turns yellow upon addition of an acidic termination solution. The density of the yellow color is proportional to the amount of CRP sample captured in the plate. The concentration of CRP is calculated by reading the absorbance at 450nm with a microplate reader.

Background: CRP is an acute-phase protein of hepatic origin, whose circulating concentrations rise in response to inflammation. It is considered a key plasma protein that can bind to Leptin, and its potential role in regulating leptin's function in obesity is crucial.

Synonyms: Pentraxin-related; pentraxin 1; C-RP; PTX1

Applications: Chicken C-reactive protein (CRP) ELISA kit (78.125-5000 pg/mL) is used to quantify CRP in serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples of chicken, providing data to support a wide range of studies.

Research Area: Inflammatory reaction; Obesity research

Specification

Sample Type: Serum; Plasma; Cell culture supernatant; Cell or tissue lysate; Other liquid samples

Detection Range: 78.125-5000 pg/mL

Sensitivity: 46.875 pg/mL

Precision (Intra-assay): CV<8%

Precision (Inter-assay): CV<10%

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at -20°C (microplate, standard) and 2-8°C (other reagents).

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