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Rat lipopolysaccharide binding protein (LBP) ELISA kit (1.56-100 ng/mL)
Cat. No.:
0126WXX-778
Assay Type:
Quantitative sandwich ELISA
Target Species:
Rat
Assay Target:
LBP
Size:
Add to basket
Product Overview
Description:
Rat lipopolysaccharide binding protein (LBP) ELISA kit (1.56-100 ng/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of LBP in rat samples with a range of 1.56-100 ng/mL and a minimum detectable dose (sensitivity) of <0.05 μg/mL. The kit is highly sensitive and easy to use.
Assay Principle:
The microplate is pre-immobilized with a rat L-Arg monoclonal antibody, and the detection antibody is a biotin-labeled polyclonal antibody. Samples and detection antibodies are added and washed with PBS or TBS, followed by the addition of avidin-peroxidase conjugates. After washing thoroughly, the TMB substrate is added for visualization. The catalytic blue color turns yellow upon the addition of acid. The color intensity is positively correlated with the testing factors in the samples.
Background:
LBP is a secreted protein primarily present in the extracellular space. Its core function is to bind bacterial lipopolysaccharide (LPS), a glycolipid found on the outer membrane of all Gram-negative bacteria. LBP is significant in obesity research because it plays a pivotal role in the link between inflammation, gut microbiota, and metabolic health. By studying LBP's mechanisms of action and its level changes, scientists can better understand how obesity leads to systemic inflammation and insulin resistance, potentially developing therapies targeting LBP or its associated signaling pathways.
Synonyms:
LPS-binding protein
Applications:
Rat lipopolysaccharide binding protein (LBP) ELISA kit (1.56-100 ng/mL) is used to quantify LBP in rat serum, plasma, cell culture supernatant, and organizations, providing data to support research in a wide range of areas, including Infection immunity, obesity, etc.
Research Area:
Infection immunity; Obesity
Specification
Sample Type:
Rat Serum; Plasma; Cell culture supernatant; Organizations
Detection Range:
1.56-100 ng/mL
Sensitivity:
<0.05 μg/mL
Precision (Intra-assay):
CV≤8%
Precision (Inter-assay):
CV≤12%
Cross-reactivity:
No significant cross-reactivity or interference was observed.
Storage:
Store at 2-8°C.
