Rat lipopolysaccharide binding protein (LBP) ELISA kit (62.5-4000 pg/mL)

Cat. No.: 0126WXX-779

Assay Type: Quantitative sandwich ELISA

Target Species: Rat

Assay Target: LBP

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Rat lipopolysaccharide binding protein (LBP) ELISA kit (62.5-4000 pg/mL)

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Product Overview

Description: Rat lipopolysaccharide binding protein (LBP) ELISA kit (62.5-4000 pg/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of LBP in rat samples with a range of 62.5-4000 pg/mL and a minimum detectable dose (sensitivity) of 37.5 pg/mL. The kit is highly sensitive and easy to use.

Assay Principle: An anti-LBP antibody is pre-coated onto 96-well plates. Biotin-conjugated anti-LBP antibodies are used for detection. Standards, samples, and detection antibodies are added sequentially and washed. HRP-streptavidin is then introduced. After washing away unbound conjugates, TMB substrate is used to visualize the reaction, turning yellow after the addition of an acidic stop solution. The resulting yellow density is proportional to the amount of LBP captured in the plate, measured at 450 nm.

Background: LBP is a secreted protein primarily present in the extracellular space. Its core function is to bind bacterial lipopolysaccharide (LPS), a glycolipid found on the outer membrane of all Gram-negative bacteria. LBP is significant in obesity research because it plays a pivotal role in the link between inflammation, gut microbiota, and metabolic health. By studying LBP's mechanisms of action and its level changes, scientists can better understand how obesity leads to systemic inflammation and insulin resistance, potentially developing therapies targeting LBP or its associated signaling pathways.

Synonyms: LPS-binding protein

Applications: Rat lipopolysaccharide binding protein (LBP) ELISA kit (62.5-4000 pg/mL) is used to quantify LBP in serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples of rat, providing data to support research in a wide range of areas, including Infection immunity, obesity, etc.

Research Area: Infection immunity; Obesity

Specification

Sample Type: Serum; Plasma; Cell culture supernatant; Cell or tissue lysate; Other liquid samples

Detection Range: 62.5-4000 pg/mL

Sensitivity: 37.5 pg/mL

Precision (Intra-assay): CV<8%

Precision (Inter-assay): CV<10%

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at -20°C (microplate, standard) and 2-8°C (other reagents).

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