Rat C-reactive protein (CRP) ELISA kit (0.156-10 ng/mL)

Cat. No.: OB0625WXX-464

Assay Type: Quantitative sandwich ELISA

Target Species: Rat

Assay Target: CRP

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Rat C-reactive protein (CRP) ELISA kit (0.156-10 ng/mL)

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Product Overview

Description: Rat C-reactive protein (CRP) ELISA kit (0.156-10 ng/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of CRP in rats with a range of 0.156-10 ng/mL and a specificity of 0.083 ng/mL.

Assay Principle: The microtiter plate provided in this kit is pre-coated with an antibody specific to CRP. Standards or samples are then added to the wells of the microtiter plate along with a biotin-conjugated polyclonal antibody specific for CRP. Horseradish peroxidase (HRP)-conjugated avidin is then added to each of the wells of the microtiter plate and incubated. TMB substrate solution is then added to each well. Only wells containing CRP, biotin-conjugated antibody, and enzyme-conjugated avidin will show a color change. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution, and the color change is then measured spectrophotometrically at 450 nm ± 2 nm. The concentration of CRP in the sample can then be determined by comparing the OD of the sample to a standard curve.

Background: CRP is an acute-phase protein of hepatic origin, whose circulating concentrations rise in response to inflammation. It is considered a key plasma protein that can bind to Leptin, and its potential role in regulating leptin's function in obesity is crucial.

Synonyms: Pentraxin-related; pentraxin 1; C-RP; PTX1

Applications: Rat C-reactive protein (CRP) ELISA kit (0.156-10 ng/mL) is used to quantify CRP in rat samples, providing data to support a wide range of studies.

Research Area: Inflammatory reaction; Obesity research

Specification

Sample Type: Rat samples

Detection Range: 0.156-10 ng/mL

Sensitivity: 0.083 ng/mL

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at 2-8°C.

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