Pigeon glucagon (GCG) ELISA kit (125-2000 pg/mL)

Cat. No.: 0126WXX-92

Assay Type: Quantitative competitive ELISA

Target Species: Pigeon

Assay Target: GCG

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Pigeon glucagon (GCG) ELISA kit (125-2000 pg/mL)

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Product Overview

Description: Pigeon glucagon (GCG) ELISA kit (125-2000 pg/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of GCG in pigeon samples with a range of 125-2000 pg/mL and a minimum detectable dose (sensitivity) of 62.5 pg/mL. The kit is highly sensitive and easy to use.

Assay Principle: This assay employs a direct competitive immunoassay technique. A capture antibody specific to GCG is pre-coated onto a microplate. GCG from the sample and a fixed amount of biotin-conjugated GCG compete for binding to the antibody. After the competitive reaction, an HRP-avidin conjugate is added to bind the biotinylated GCG. A substrate solution is introduced, and color intensity is measured spectrophotometrically. The intensity is inversely proportional to the GCG concentration, as higher sample GCG levels result in less bound biotin-conjugated GCG and a reduced signal.

Background: Glucagon is a hormone secreted by A cells of the islets of Langerhans, playing a pivotal role in glucose metabolism and homeostasis. Its primary function is to regulate blood glucose levels by promoting gluconeogenesis and inhibiting glycolysis. The dynamic equilibrium between glucagon and insulin is crucial for maintaining healthy metabolism. The detection of glucagon and analysis of its associated pathways provide key insights into understanding and treating obesity.

Synonyms: GLP1; GLP2; GRPP; Glicentin-related polypeptide; Glucagen; Oxyntomodulin; Incretin hormone; GC

Applications: Pigeon glucagon (GCG) ELISA kit (125-2000 pg/mL) is used to quantify GCG in serum, plasma, and tissue homogenates of pigeon, providing data to support research in a wide range of areas, including endocrinology, obesity, metabolism, and others.

Research Area: Endocrinology; Obesity; Metabolism

Specification

Sample Type: Serum; Plasma; Tissue homogenates

Detection Range: 125-2000 pg/mL

Sensitivity: 62.5 pg/mL

Precision (Intra-assay): CV<8%

Precision (Inter-assay): CV<10%

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at 2-8°C.

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