Rabbit adiponectin (ADP) ELISA kit

Cat. No.: OB0525WXX-273

Assay Type: Quantitative sandwich ELISA

Target Species: Rabbit

Assay Target: ADP

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Product Overview

Description: Rabbit adiponectin (ADP) ELISA kit is a ready-to-use ELISA kit for analyzing rabbit ADP levels with high sensitivity and excellent specificity.

Assay Principle: An antibody specific for ADP has been pre-coated onto a microtiter plate. Standards and samples are pipetted into the wells, and any ADP present is bound by the immobilized antibody. After removing unbound material, a biotin-conjugated antibody specific for ADP is added to the wells. After washing, streptavidin-conjugated HRP is added to the wells. After washing to remove unbound avidin-enzyme reagent, a substrate solution is added to the wells, and the color is developed proportionally to the amount of ADP bound in step one. The color development is stopped, and the intensity of the color is measured.

Background: ADP is an important adipokine involved in the regulation of fat metabolism and insulin sensitivity, exhibiting antidiabetic, anti-atherosclerotic, and anti-inflammatory properties. It increases glucose utilization and fatty acid burning, stimulates AMPK phosphorylation and activation in liver and skeletal muscle, and may play a role in cell growth, angiogenesis, and tissue remodeling.

Synonyms: ADIPOQ; ACDC; APM1; Adipocyte C1q and collagen domain-containing protein; ACRP30; Adipose most abundant gene transcript 1 protein; apM-1; Gelatin-binding protein; Adiponectin

Applications: Rabbit adiponectin (ADP) ELISA kit is designed for the in vitro quantitative analysis of ADP levels in rabbit serum, plasma, and other biological fluid samples.

Research Area: Hormone research; Lipid metabolism; Cell differentiation; Obesity

Specification

Sample Type: Serum; Plasma; Other biological fluids

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at 2-8°C.

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