Human adiponectin (ADP) ELISA kit (0.156-10 ng/mL, 0.05 ng/mL)

Cat. No.: OB0525WXX-151

Assay Type: Quantitative sandwich ELISA

Target Species: Human

Assay Target: ADP

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Human adiponectin (ADP) ELISA kit (0.156-10 ng/mL, 0.05 ng/mL)

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Product Overview

Description: Human adiponectin (ADP) ELISA kit (0.156-10 ng/mL, 0.05 ng/mL) is a ready-to-use ELISA kit for analyzing human ADP levels with a detection range of 0.156-10 ng/mL and a sensitivity of 0.05 ng/mL.

Assay Principle: The kit is based on the double antibody sandwich ELISA technique. The pre-coated antibody is anti-canine ADP monoclonal antibody, and the detection antibody is biotinylated polyclonal antibody. The samples and biotinylated antibodies are added into the wells of the ELISA plate separately, and then rinsed with PBS or TBS. Avidin-peroxidase conjugate is then sequentially added to the wells. The enzyme conjugates are thoroughly washed with PBS or TBS and then colored with TMB substrate, which reacts with the peroxidase activity to produce a blue product, which finally turns yellow upon addition of the termination solution (color developing reagent C). The intensity of the color is positively correlated with the amount of target analyte in the sample.

Background: ADP is an important adipokine involved in the regulation of fat metabolism and insulin sensitivity, exhibiting antidiabetic, anti-atherosclerotic, and anti-inflammatory properties. It increases glucose utilization and fatty acid burning, stimulates AMPK phosphorylation and activation in liver and skeletal muscle, and may play a role in cell growth, angiogenesis, and tissue remodeling.

Synonyms: ADIPOQ; ACDC; APM1; Adipocyte C1q and collagen domain-containing protein; ACRP30; Adipose most abundant gene transcript 1 protein; apM-1; Gelatin-binding protein; Adiponectin

Applications: Human adiponectin (ADP) ELISA kit (0.156-10 ng/mL, 0.05 ng/mL) is designed for the in vitro quantitative analysis of ADP levels in human serum, plasma, cell culture supernatant, organizations in the natural and recombinant ADP concentration samples.

Research Area: Hormone research; Lipid metabolism; Cell differentiation; Obesity

Specification

Sample Type: Human serum; Plasma; Cell culture supernatant; Organizations in the natural and recombinant ADP concentration

Detection Range: 0.156-10 ng/mL

Sensitivity: 0.05 ng/mL

Precision (Intra-assay): CV≤8%

Precision (Inter-assay): CV≤12%

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at 2-8°C.

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