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Rat agouti-related protein (AGRP) ELISA kit (9.4-600 pg/mL)
Cat. No.:
OB0625WXX-007
Assay Type:
Quantitative sandwich ELISA
Target Species:
Rat
Assay Target:
AGRP
Size:
Add to basket
Product Overview
Description:
Rat agouti-related protein (AGRP) ELISA kit (9.4-600 pg/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of AGRP in rat with a range of 9.4-600 pg/mL and a specificity of 4.1 pg/mL.
Assay Principle:
The microtiter plate provided in this kit is pre-coated with an antibody specific for AGRP. Standards or samples are then added to the appropriate microtiter plate wells along with a biotin-conjugated antibody specific for AGRP. Avidin conjugated to horseradish peroxidase (HRP) is then added to each microtiter plate well and incubated. Upon addition of TMB substrate solution, only the wells containing AGRP, biotin-conjugated antibody, and enzyme-conjugated avidin will show a color change. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution, and the color change is then measured spectrophotometrically at 450 nm ± 10 nm. The concentration of AGRP in the sample can then be determined by comparing the OD of the sample to a standard curve.
Background:
AGRP plays a role in body weight homeostasis. It participates in the control of feeding behavior via the central melanocortin system and is associated with obesity. By inhibiting the cAMP production mediated by the stimulation of melanocortin receptors in the hypothalamus and adrenal gland, it acts as an α-MSH antagonist.
Synonyms:
Agouti related protein; AGRT; ASIP2; ART
Applications:
Rat agouti-related protein (AGRP) ELISA kit (9.4-600 pg/mL) is used to quantify AGRP in serum, plasma, tissue homogenates, and other biological fluid samples of rat, providing data to support a wide range of studies.
Research Area:
Signal transduction; Metabolic pathway; Neuro science; Hormone metabolism; Obesity research
Specification
Sample Type:
Serum; Plasma; Tissue homogenates; Other biological fluids
Detection Range:
9.4-600 pg/mL
Sensitivity:
4.1 pg/mL
Precision (Intra-assay):
CV<10%
Precision (Inter-assay):
CV<12%
Cross-reactivity:
No significant cross-reactivity or interference was observed.
Storage:
Store at 2-8°C.
