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Human adiponectin (ADP) ELISA kit (1.56-100 ng/mL, <60 pg/mL)
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Cat. No.:
OB0525WXX-217
Synonyms:
Human ADP ELISA kit; Human adiponectin (ADP); Human adiponectin; Adiponectin (ADP); Human ADP; ADP ELISA kit
Size:
96T
Specification
Description:
Human adiponectin (ADP) ELISA kit (1.56-100 ng/mL, <60 pg/mL) is a ready-to-use ELISA kit for analyzing human ADP levels with a detection range of 1.56-100 ng/mL. Its sensitivity is less than 60 pg/mL.
Background:
ADP is an important adipokine involved in the regulation of fat metabolism and insulin sensitivity, exhibiting antidiabetic, anti-atherosclerotic, and anti-inflammatory properties. It increases glucose utilization and fatty acid burning, stimulates AMPK phosphorylation and activation in liver and skeletal muscle, and may play a role in cell growth, angiogenesis, and tissue remodeling.
Formula Weight:
26,414 Da
Assay Principle:
The detection antibody is a biotinylated antibody specific for ADP. The capture antibody is a mouse monoclonal antibody, and the detection antibody is a goat polyclonal antibody. To detect human ADP, standards and samples are added to the wells, followed by biotinylated detection antibody. The wells are washed with PBS or TBS buffer, and ABC-HRP is added. Unbound ABC-HRP is washed with PBS or TBS buffer, and TMB is added. TMB is an HRP substrate that catalyzes the production of a blue product, which turns yellow upon addition of an acidic termination solution. The absorbance of the yellow product at 450 nm is linearly proportional to the human ADP in the sample. The absorbance of the yellow product in each well is read using a plate reader, and the readings from the sample wells are compared to a standard curve to determine the concentration of human ADP in the sample.
Assay Target:
ADP
Target Species:
Human
Research Area:
Hormone research; Lipid metabolism; Cell differentiation; Obesity
Sample Type:
Cell culture supernatants; Serum; Plasma (Heparin; edta); Urine
Detection Range:
1.56-100 ng/mL
Sensitivity:
<60 pg/mL
Cross-reactivity:
No significant cross-reactivity or interference was observed.
Stability:
12 Months
Applications:
Human adiponectin (ADP) ELISA kit (1.56-100 ng/mL, <60 pg/mL) is designed for the in vitro quantitative analysis of ADP levels in human cell culture supernatants, serum, plasma (heparin, edta), and urine samples.
Storage:
Store at 2-8°C.