Human cytochrome c oxidase subunit 7C (COX7C) ELISA kit

Cat. No.: OB0625WXX-390

Assay Type: Quantitative competitive ELISA

Target Species: Human

Assay Target: COX7C

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Human cytochrome c oxidase subunit 7C (COX7C) ELISA kit

INQUIRY Datasheet MSDS

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Product Overview

Description: Human cytochrome c oxidase subunit 7C (COX7C) ELISA kit is an ELISA-based in vitro research tool designed specifically for the quantitative detection of COX7C in human with a specificity of 1.0 ng/mL.

Assay Principle: The assay sample and buffer are incubated together with COX7C-HRP conjugate in a pre-coated plate for one hour. At the end of the incubation period, the wells are decanted and washed five times. The wells are then incubated with HRP enzyme substrate. The products of the enzyme-substrate reaction form a blue color complex. Finally, the reaction is stopped by adding a stop solution, which turns the solution yellow. The color intensity is measured spectrophotometrically at 450 nm on a microplate reader. The color intensity is inversely proportional to the concentration of COX7C because the COX7C in the sample competes with the COX7C-HRP conjugate for the binding site of the anti-COX7C antibody. Since there are a limited number of binding sites, as more sites are occupied by COX7C in the sample, fewer sites remain to bind the COX7C-HRP conjugate. A standard curve is plotted between color intensity (O.D.) and standard concentration. The concentration of COX7C in each sample is derived from this standard curve.

Background: COX7C is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport.

Synonyms: COXVIIc; Cytochrome c oxidase subunit 7C, mitochondrial; Cytochrome c oxidase subunit VIIc; Cytochrome c oxidase polypeptide VIIc; Cytochrome-C oxidase chain VIIc

Applications: Human cytochrome c oxidase subunit 7C (COX7C) ELISA kit is an important tool for the quantitative detection of COX7C in various biological samples (serum, plasma, cell culture supernatants, body fluid, tissue homogenate) of human.

Research Area: Metabolic pathway; Obesity research

Specification

Sample Type: Serum; Plasma; Cell culture supernatants; Body fluid; Tissue homogenate

Sensitivity: 1.0 ng/mL

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at 2-8°C.

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