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Vitamin B12 test kit

Rat estrogen receptor (ER) ELISA kit (0.5-150 ng/mL)

Cat. No.: OB0625WXX-721
Assay Type: Quantitative sandwich ELISA
Target Species: Rat
Assay Target: ER
Size:
Rat estrogen receptor (ER) ELISA kit (0.5-150 ng/mL)
INQUIRY Datasheet MSDS
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Product Overview

Description: Rat estrogen receptor (ER) ELISA kit (0.5-150 ng/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of ER in rat with a range of 0.5-150 ng/mL and a specificity of 0.28 ng/mL.
Assay Principle: A rat ER antibody is used to coat the plate. ER from the sample is added to bind to the antibody coated on the wells. Biotinylated rat ER antibody is then added and binds to the ER in the sample. Streptavidin-HRP is then added and binds to the biotinylated ER antibody. After incubation, unbound streptavidin-HRP is washed out in a wash step. A substrate solution is then added, and the color development is proportional to the amount of murine ER. The reaction is terminated by the addition of an acidic termination solution, and the absorbance is measured at 450 nm.
Background: ER is a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. Estrogens and their receptors play roles in sexual development, reproductive functions, bone, and other tissues. ERs are associated with various pathological processes.
Synonyms: Estradiol receptor; Oestrogen receptor; Receptors, Estrogen
Applications: Rat estrogen receptor (ER) ELISA kit (0.5-150 ng/mL) is used to quantify ER in serum, plasma, cell culture supernates, cell lysates, tissue homogenate samples of rat, providing data to support a wide range of studies.
Research Area: Endocrinology

Specification

Sample Type: Serum; Plasma; Cell culture supernates; Cell lysates; Tissue homogenates
Detection Range: 0.5-150 ng/mL
Sensitivity: 0.28 ng/mL
Cross-reactivity: No significant cross-reactivity or interference was observed.
Storage: Store at 2-8°C.

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