Cat adiponectin (ADP) ELISA kit (1.25-40 mμg/mL)

Cat. No.: OB0525WXX-291

Assay Type: Quantitative sandwich ELISA

Target Species: Cat

Assay Target: ADP

Size:

Cat adiponectin (ADP) ELISA kit (1.25-40 mμg/mL)

INQUIRY Datasheet MSDS

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Product Overview

Description: Cat adiponectin (ADP) ELISA kit (1.25-40 mμg/mL) is a ready-to-use ELISA kit for analyzing cat ADP levels with a detection range of 1.25-40 mμg/mL and a sensitivity of 0.1 mμg/mL.

Assay Principle: The wells of the plate are coated with purified bovine ADP antibody to form a solid-phase antibody. Then, ADP and HRP-labeled ADP antibody are added to the wells to form an antibody-antigen-antibody-enzyme complex. After a complete-ish, TMB substrate is added and the TMB substrate turns blue when catalyzed by the enzyme HRP. Then the reaction is terminated by the addition of sulfuric acid, followed by spectrophotometric analysis at 450 nm. The concentration of ADP in the sample is then determined by comparing the OD of the sample with the standard curve.

Background: ADP is an important adipokine involved in the regulation of fat metabolism and insulin sensitivity, exhibiting antidiabetic, anti-atherosclerotic, and anti-inflammatory properties. It increases glucose utilization and fatty acid burning, stimulates AMPK phosphorylation and activation in liver and skeletal muscle, and may play a role in cell growth, angiogenesis, and tissue remodeling.

Synonyms: ADIPOQ; ACDC; APM1; Adipocyte C1q and collagen domain-containing protein; ACRP30; Adipose most abundant gene transcript 1 protein; apM-1; Gelatin-binding protein; Adiponectin

Applications: Cat adiponectin (ADP) ELISA kit (1.25-40 mμg/mL) is designed for the in vitro quantitative analysis of ADP levels in cat serum, plasma, tissue homogenate, feces, and urine samples.

Research Area: Hormone research; Lipid metabolism; Cell differentiation; Obesity

Specification

Sample Type: Serum; Plasma; Tissue homogenate; Feces; Urine

Detection Range: 1.25-40 mμg/mL

Sensitivity: 0.1 mug/mL

Precision (Intra-assay): CV<15%

Precision (Inter-assay): CV<15%

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at 2-8°C.

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