Mouse Iinsulin (INS) ELISA kit (0.313-20 ng/mL)

Cat. No.: 0126WXX-593

Assay Type: Quantitative sandwich ELISA

Target Species: Mouse

Assay Target: INS

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Mouse Iinsulin (INS) ELISA kit (0.313-20 ng/mL)

INQUIRY Datasheet MSDS

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Product Overview

Description: Mouse insulin (INS) ELISA kit (0.313-20 ng/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of INS in mice samples with a range of 0.313-20 ng/mL and a minimum detectable dose (sensitivity) of 0.188 ng/mL. The kit is highly sensitive and easy to use.

Assay Principle: This assay employs a highly specific Sandwich-ELISA method, utilizing a microtiter plate pre-coated with a capture antibody specific to INS. During the procedure, INS in the standards or samples binds to the immobilized antibody, followed by the sequential addition of a biotinylated detection antibody and an Avidin-HRP conjugate to form a stable immune complex. After thorough washing to eliminate free components, a substrate solution is introduced, producing a blue color in wells containing the target analyte; the reaction is then terminated with sulphuric acid, shifting the color to yellow. The optical density (O.D.) is measured at 450 nm, where the signal intensity is directly proportional to the INS concentration, allowing for accurate quantification via comparison with a standard curve.

Background: INS is a hormone composed of an A chain and a B chain linked by two disulfide bonds. Secreted by the pancreas, it functions as a key regulator of metabolism within the body. Its primary role is to lower blood glucose levels by promoting glucose uptake into cells. INS is fundamentally linked to obesity through its role in energy metabolism and its relationship with insulin resistance. By targeting the insulin receptor signaling pathway and the diverse metabolic processes it regulates, researchers can develop novel anti-obesity therapies.

Synonyms: Pancreatic insulin

Applications: Mouse Iinsulin (INS) ELISA kit (0.313-20 ng/mL) is used to quantify INS in serum, plasma, and biological fluids of mouse, providing data to support research in a wide range of areas, including metabolic pathway, endocrinology, hormone metabolism, obesity, etc.

Research Area: Metabolic pathway; Endocrinology; Hormone metabolism; Obesity

Specification

Sample Type: Serum; Plasma; Biological fluids

Detection Range: 0.313-20 ng/mL

Sensitivity: 0.188 ng/mL

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at 4°C.

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