LipoKnoxa™ Human RAI1 shRNA Lentiviral Particle (Silencing)

Sub Cat. No.	TargetSeq	Region	Inquiry
V0126XX488-1	CTGCTCGCCAAGGACTATTAT	CDS	Inquiry
V0126XX488-2	ATAAGAGGCTGCCGTAGTAAT	CDS	Inquiry
V0126XX488-3	GCCTCGTTATATAGTGTATAT	3' UTR	Inquiry
V0126XX488-4	Other		Inquiry

Product Overview

Description: LipoKnoxa™ Human RAI1 shRNA Lentiviral Particle (Silencing) targets the gene linked to Smith-Magenis syndrome, involving truncal obesity and hyperphagia. Silencing RAI1 through our dual-strategy shRNA platform (U6 and miR30) helps researchers model transcriptional regulation in energy balance. To ensure the highest level of biosafety, all products undergo total quality control screening, ensuring high functional titers and the absence of microbial contaminants.

Production Cell Line: HEK293T

Promoter: U6; CMV; EF1α; CAG; UBC

Product Availability: Produced Upon Order

Specification

Titer Test: qPCR

Insert Verification: Comprehensive sequencing and PCR analyses were performed to verify the accurate genomic sequence of all viral preparations.

Sterility Test: Confirmed sterile via rigorous microbial analysis; free of bacterial and fungal contamination.

Mycoplasma Test: Rigorous quality control testing has confirmed the absence of mycoplasma contamination in this viral preparation.

Other QC: Customized supplementary testing and in vitro/in vivo assessments are available to verify post-silencing gene expression and biological functionality, ensuring the viral preparations meet the specific potency requirements of your gene interference project.

Storage: Store at -80°C for long-term preservation. Immediate transfer upon delivery is required to prevent loss of viral activity.

Stability: Shelf life: 6–12 months at -80°C (extended stability up to 2 years). Post-thaw: Stable at 4°C for 2–3 weeks without significant degradation of functional titers.

Shipping Condition: Products are delivered on dry ice to maintain the cold chain. Upon arrival, please ensure the presence of dry ice and transfer the vials immediately to -80°C storage.

Handling Notes: Avoid repeated freeze-thaw cycles. Aliquot into low-protein-binding tubes immediately after receipt to maintain optimal activity. For safety and contamination control, all viral handling must be performed inside a biosafety cabinet.

Intended Use: This product is intended for research use only and is not for use in diagnosis or therapeutic applications.

Product Disclaimer: We ensure product integrity through rigorous QC, but we do not guarantee results in specific research contexts. Proper storage, handling, and total compliance with biosafety laws and safety protocols remain the sole responsibility of the user.

Target Profile

Gene Name: RAI1

Full Name: Retinoic acid induced 1

Gene Symbol: SMS; SMCR

Gene ID: 10743

RefSeq ID-1: NP_109590.3

RefSeq ID-2: NM_030665.3

Summary: RAI1 is located within the Smith–Magenis syndrome region on chromosome 17 and is highly expressed in neuronal tissues. The protein contains a polymorphic polyglutamine tract and is involved in transcriptional regulation. Altered RAI1 dosage is associated with hyperphagia, abnormal circadian rhythm, and syndromic obesity. In mouse models, neuronal expression of RAI1 is regulated by retinoic acid, supporting its role in neurodevelopmental and metabolic pathways.

LipoKnoxa™ Human RAI1 shRNA Lentiviral Particle (Silencing)

Product Overview

Specification

Target Profile

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