AdipoUpX™ Human AFF4 Lentiviral Particle (Overexpression)

Product Overview

Description: AdipoUpX™ Human AFF4 Lentiviral Particle (Overexpression) is a ready-to-use, high-titer vector particle designed for the efficient and stable overexpression of the human AFF4 gene. As a central component of the super elongation complex, its stable integration into the host genome allows for in-depth research into the transcriptional regulation of adipogenesis. To ensure experimental safety and reproducibility, the product undergoes comprehensive quality control, including titer and mycoplasma testing, while offering flexible options for reporter gene selection.

Production Cell Line: HEK293T

Promoter: Ubi; CMV; EF1a; Others

Product Availability: Produced Upon Order

Specification

Titer Test: qPCR

Insert Verification: PCR analysis was performed to verify the complete genomic sequence of all viral vector preparations.

Sterility Test: This product was certified sterile following successful microbial growth analysis.

Mycoplasma Test: The production quality control confirmed the absence of any mycoplasmal agents.

Other QC: This comprehensive process includes customized supplementary testing alongside in vitro and in vivo transduction assessments to elucidate transgene expression and functionality.

Storage: To maintain product integrity, immediate transfer of the vector to a -80 °C is essential following its receipt.

Stability: Continuous storage at -80°C ensures excellent long-term stability for 6-12 months. Once thawed, the product's working solution should be refrigerated at 4°C and used within 2-3 weeks.

Shipping Condition: Our lentivirus viral products are shipped using dry ice.

Handling Notes: Ensure all operations are performed within a biosafety cabinet; upon receiving the vector, immediately aliquot it into low-protein-binding tubes and store according to specified conditions, thus avoiding the titer loss caused by sensitivity to temperature fluctuations and freeze-thaw cycles.

Intended Use: This product is intended for research use only and is not for use in diagnosis or therapeutic applications.

Product Disclaimer: Given the diversity and complexity of experimental conditions, we do not guarantee product performance or experimental results in any specific application, despite our company's commitment to product quality and the rigorous internal QC inspections all products have undergone. Ultimate responsibility remains with the user for all operational processes, including storage, handling, and compliance with safety and biosafety protocols.

Target Profile

Gene Name: AFF4

Full Name: ALF transcription elongation factor 4

Gene Symbol: MCEF; CHOPS; AF5Q31

Gene ID: 27125

RefSeq ID-1: NP_055238.1

RefSeq ID-2: NM_014423.4

Summary: The protein encoded by AFF4 is a member of the AF4 family of transcription factors, recognized for its involvement in leukemogenesis. Crucially, AFF4 is an integral subunit of the Positive Transcription Elongation Factor b (P-TEFb) complex, which is responsible for regulating the elongation phase of gene transcription. Its function is often disrupted in cancer; specifically, chromosomal translocations involving AFF4 and the MLL gene on chromosome 11 are characteristic of certain aggressive forms of infant acute lymphoblastic leukemia.

AdipoUpX™ Human AFF4 Lentiviral Particle (Overexpression)

Product Overview

Specification

Target Profile

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