AdipoUpX™ Human PHIP Lentiviral Particle (Overexpression)

Product Overview

Description: AdipoUpX™ Human PHIP Lentiviral Particle (Overexpression) is a professionally engineered vector particle providing efficient and long-lasting overexpression of the human PHIP gene. PHIP acts as a molecular bridge between insulin signaling and POMC expression, making this stable integration system an ideal tool for validating genetic links in obesity. All viral particle products undergo comprehensive quality control (titer, sterility, and mycoplasma detection) to ensure safety, and the modular vector design offers high flexibility for custom markers.

Production Cell Line: HEK293T

Promoter: Ubi; CMV; EF1a; Others

Product Availability: Produced Upon Order

Specification

Titer Test: qPCR

Insert Verification: PCR analysis was performed to verify the complete genomic sequence of all viral vector preparations.

Sterility Test: This product was certified sterile following successful microbial growth analysis.

Mycoplasma Test: The production quality control confirmed the absence of any mycoplasmal agents.

Other QC: This comprehensive process includes customized supplementary testing alongside in vitro and in vivo transduction assessments to elucidate transgene expression and functionality.

Storage: To maintain product integrity, immediate transfer of the vector to a -80 °C is essential following its receipt.

Stability: Continuous storage at -80°C ensures excellent long-term stability for 6-12 months. Once thawed, the product's working solution should be refrigerated at 4°C and used within 2-3 weeks.

Shipping Condition: Our lentivirus viral products are shipped using dry ice.

Handling Notes: Ensure all operations are performed within a biosafety cabinet; upon receiving the vector, immediately aliquot it into low-protein-binding tubes and store according to specified conditions, thus avoiding the titer loss caused by sensitivity to temperature fluctuations and freeze-thaw cycles.

Intended Use: This product is intended for research use only and is not for use in diagnosis or therapeutic applications.

Product Disclaimer: Given the diversity and complexity of experimental conditions, we do not guarantee product performance or experimental results in any specific application, despite our company's commitment to product quality and the rigorous internal QC inspections all products have undergone. Ultimate responsibility remains with the user for all operational processes, including storage, handling, and compliance with safety and biosafety protocols.

Target Profile

Gene Name: PHIP

Full Name: PHIP subunit of CUL4-Ring ligase complex

Gene Symbol: ndrp; BRWD2; DIDOD; RepID; WDR11; DCAF14; CHUJANS

Gene ID: 55023

RefSeq ID-1: NP_060404.4

RefSeq ID-2: NM_017934.6

Summary: PHIP encodes a protein primarily recognized for its ability to bind to Insulin Receptor Substrate 1 (IRS1), thereby playing a role in regulating the translocation of glucose transporters within skeletal muscle cells. This protein may also contribute to the growth and survival of pancreatic beta cells. Beyond metabolism, PHIP has oncogenic relevance: elevated gene copy number is linked to increased melanoma severity, and the protein itself is postulated to promote melanoma metastasis in patients.

AdipoUpX™ Human PHIP Lentiviral Particle (Overexpression)

Product Overview

Specification

Target Profile

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