AdipoUpX™ Human IRS2 Lentiviral Particle (Overexpression)

Product Overview

Description: AdipoUpX™ Human IRS2 Lentiviral Particle (Overexpression) is a high-titer system for the specific and stable expression of the human IRS2 gene. As a critical adapter for insulin and IGF1 signaling, its stable overexpression is essential for investigating the protection of beta-cell mass and hepatic insulin sensitivity. The lentivirus characteristic of genome integration ensures the establishment of reliable cell models for high-fidelity signaling studies. Comprehensive quality control is performed to ensure both product safety and the reproducibility of results.

Production Cell Line: HEK293T

Promoter: Ubi; CMV; EF1a; Others

Product Availability: Produced Upon Order

Specification

Titer Test: qPCR

Insert Verification: PCR analysis was performed to verify the complete genomic sequence of all viral vector preparations.

Sterility Test: This product was certified sterile following successful microbial growth analysis.

Mycoplasma Test: The production quality control confirmed the absence of any mycoplasmal agents.

Other QC: This comprehensive process includes customized supplementary testing alongside in vitro and in vivo transduction assessments to elucidate transgene expression and functionality.

Storage: To maintain product integrity, immediate transfer of the vector to a -80 °C is essential following its receipt.

Stability: Continuous storage at -80°C ensures excellent long-term stability for 6-12 months. Once thawed, the product's working solution should be refrigerated at 4°C and used within 2-3 weeks.

Shipping Condition: Our lentivirus viral products are shipped using dry ice.

Handling Notes: Ensure all operations are performed within a biosafety cabinet; upon receiving the vector, immediately aliquot it into low-protein-binding tubes and store according to specified conditions, thus avoiding the titer loss caused by sensitivity to temperature fluctuations and freeze-thaw cycles.

Intended Use: This product is intended for research use only and is not for use in diagnosis or therapeutic applications.

Product Disclaimer: Given the diversity and complexity of experimental conditions, we do not guarantee product performance or experimental results in any specific application, despite our company's commitment to product quality and the rigorous internal QC inspections all products have undergone. Ultimate responsibility remains with the user for all operational processes, including storage, handling, and compliance with safety and biosafety protocols.

Target Profile

Gene Name: IRS2

Full Name: Insulin receptor substrate 2

Gene Symbol: IRS-2

Gene ID: 8660

RefSeq ID-1: NP_003740.2

RefSeq ID-2: NM_003749.2

Summary: IRS2 serves as a vital molecular bridge in the insulin signaling cascade. Acting as an adaptor protein, it links activated receptor tyrosine kinases (like the insulin receptor or IGF-1 receptor) to downstream intracellular effectors. It is primarily regulated through phosphorylation. Dysfunction in the IRS2 pathway is a central mechanism in the development of insulin resistance, as it prevents the cell from correctly interpreting metabolic signals from the bloodstream.

AdipoUpX™ Human IRS2 Lentiviral Particle (Overexpression)

Product Overview

Specification

Target Profile

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