LipoKnoxa™ Human CASP1 shRNA Lentiviral Particle (Silencing)

Sub Cat. No.	TargetSeq	Region	Inquiry
V0126XX621-1	CACACGTCTTGCTCTCATTAT	CDS	Inquiry
V0126XX621-2	CTACAACTCAATGCAATCTTT	CDS	Inquiry
V0126XX621-3	CCAGATATACTACAACTCAAT	CDS	Inquiry
V0126XX621-4	Other		Inquiry

Product Overview

Description: LipoKnoxa™ Human CASP1 shRNA Lentiviral Particle (Silencing) targets caspase-1, the central enzyme of the inflammasome that processes cytokines like IL-1β in obesity. Silencing CASP1 through our dual-strategy shRNA platform (U6 and miR30) helps researchers model chronic metabolic inflammation. Every preparation undergoes total quality control verification—covering functional activity, sequence identity, and sterility—ensuring high biosafety and consistent performance in immune-metabolic studies.

Production Cell Line: HEK293T

Promoter: U6; CMV; EF1α; CAG; UBC

Product Availability: Produced Upon Order

Specification

Titer Test: qPCR

Insert Verification: Comprehensive sequencing and PCR analyses were performed to verify the accurate genomic sequence of all viral preparations.

Sterility Test: Confirmed sterile via rigorous microbial analysis; free of bacterial and fungal contamination.

Mycoplasma Test: Rigorous quality control testing has confirmed the absence of mycoplasma contamination in this viral preparation.

Other QC: Customized supplementary testing and in vitro/in vivo assessments are available to verify post-silencing gene expression and biological functionality, ensuring the viral preparations meet the specific potency requirements of your gene interference project.

Storage: Store at -80°C for long-term preservation. Immediate transfer upon delivery is required to prevent loss of viral activity.

Stability: Shelf life: 6–12 months at -80°C (extended stability up to 2 years). Post-thaw: Stable at 4°C for 2–3 weeks without significant degradation of functional titers.

Shipping Condition: Products are delivered on dry ice to maintain the cold chain. Upon arrival, please ensure the presence of dry ice and transfer the vials immediately to -80°C storage.

Handling Notes: Avoid repeated freeze-thaw cycles. Aliquot into low-protein-binding tubes immediately after receipt to maintain optimal activity. For safety and contamination control, all viral handling must be performed inside a biosafety cabinet.

Intended Use: This product is intended for research use only and is not for use in diagnosis or therapeutic applications.

Product Disclaimer: We ensure product integrity through rigorous QC, but we do not guarantee results in specific research contexts. Proper storage, handling, and total compliance with biosafety laws and safety protocols remain the sole responsibility of the user.

Target Profile

Gene Name: CASP1

Full Name: Caspase 1

Gene Symbol: ICE; P45; IL1BC

Gene ID: 834

RefSeq ID-1: NP_001214.1

RefSeq ID-2: NM_001223.5

Summary: CASP1 encodes caspase-1, a cysteine-aspartic acid protease involved in both apoptosis and inflammation. It is best known for processing inactive pro–interleukin-1β into its active form, thereby promoting inflammatory responses. In obesity, CASP1-mediated inflammasome activation in adipose tissue contributes to chronic low-grade inflammation and insulin resistance. The gene produces multiple isoforms through alternative splicing.

LipoKnoxa™ Human CASP1 shRNA Lentiviral Particle (Silencing)

Product Overview

Specification

Target Profile

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